Validity of peripheral blood smear as a screening tool for Sickle Cell Anemia: Comparison with electrophoresis | [Rawal Medical Journal • 2023]

Author(s):

1. Durr-e-Sameen Kamran: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

2. Shahneela Siraj: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

3. Imran Ishaq: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

4. Sumaira Riffat: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

5. Aisha Abdul Haq: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

6. Afshan Mehboob Khan: Department of Pathology, Dow Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, United Medical and Dental College and Jinnah Sindh Medical University,Sindh,Pakistan

Abstract:

Objective: To evaluate the diagnostic accuracy of peripheral smear morphology as a screening tool for Sickle cell trait/disease by comparing its results with hemoglobin electrophoresis. Methodology: Two hundred persons of Sheedi/ Makrani caste were included in the study during the period of one year from Lyari General Hospital, Karachi. Peripheral smears were prepared and electrophoresis was performed on their blood samples. Analysis was performed on SPSS 16. Results: Peripheral smear was found to be very sensitive and specific for Sickle cell disease, it was also found to be 100% specific for Sickle cell trait but it was not a very sensitive tool for Sickle trait . Conclusion: CBC and smear morphology can be utilized for Sickle screening in high-risk areas, which can further be backed up by more sophisticated techniques in doubtful cases.

Page(s): 47-49

DOI: DOI not available

Published: Journal: Rawal Medical Journal, Volume: 48, Issue: 1, Year: 2023

Keywords:

Complete Blood Count , sickle cell disease , peripheral smear morphology , sickle cell trait , hemoglobin electrophoresis

References:

[1] Piel FB,Steinberg MH,Rees DC .2017 .Sickle cell disease. New Engl J Med, 376 : 1561-73.

[2] Piel FB,Patil AP,Howes RE .2013 .Global epidemiology of sickle hemoglobin in neonates: a contemporary geostatistical model-based map and population estimates. Lancet, 381 : 142-51.

[3] Piel FB,Tatem AJ,Huang Z,Gupta S,Williams TN,Weatherall DJ .2014 .Global migration and the changing distribution of sickle haemoglobin: a quantitative study of temporal trends between 1960 and 2000. Lancet Glob Health, 2 : e80-9.

[4] Ojodu J,Hulihan MM,Pope SN,United States .2010 .Centers for Disease Control and Prevention (CDC). Incidence of sickle cell trait-. MMWR Morb Mortal Wkly Rep, 63 : 1155-1158.

[5] Creary M,Williamson D,Kulkarni R .2007 .Sickle cell disease: current activities, public health implications, and future directions. J Womens Health, 16 : 575-82.

[6] Makani J,Williams TN,Marsh K. .2007 .Sickle cell disease in Africa: Burden and research priorities. Ann Trop Med Parasitol, 101 : 3-14.

[7] Wilson CI,Hopkins PL,Cabello-Inchausti B,Melnick SJ,Robinson MJ SJ .2000 .The peripheral blood smear in patients with sickle cell trait: a morphologic observation. Lab Med, 31 : 445-7.

[8] Nia J,Lam WC,Kleinman DM,Kirby M,Liu ES,Eng KT .2003 .Retinopathy in sickle cell trait: does it exist?. Can J Ophthalmol, 38 : 46-51.

[9] Nia J,Lam WC,Kleinman DM,Kirby M,Liu ES,Eng KT .2003 .Retinopathy in sickle cell trait: does it exist?. Can J Ophthalmol, 38 : 46-51.

[10] Chakravorty S,Williams TN .2015 .Sickle cell disease: a neglected chronic disease of increasing global health importance. Arch Dis Child, 100 : 48-53.

[11] Ware RE,de Montalembert M,Tshilolo L,Abboud MR .2017 .Sickle cell disease. Lancet, 390 : 311-23.

[12] Weatherall D,Akinyanju O,Fucharoen S,Olivieri N,Musgrove P .2006 .Inherited disorders of hemoglobin. Disease Control Priorities in Developing Countries. 2nd edition, : .

[13] Kazmi KA,Rab SM .1990 .Sickle cell anaemia in Pakistan. Br J Clin Pract, 44 : 503-5.

[14] Murtaza F,Mustafa T,Awan R .2015 .Child health inequalities and its dimensions in Pakistan. J Fam Comm Med, 22 : 169-72.

[15] Badrick T .2013 .Evidence-based laboratory medicine. Clin Biochem Rev, 34 : 43-8.

[16] Ghani R,Manji MA,Ahmed N. .2002 .Hemoglobinopathies among five major ethnic groups in Karachi, Pakistan. Southeast Asian J Trop Med, 33 : 855-61.

[17] Hamali HA,Saboor M.,Undiagnosed M. .2019 .Hemoglobinopathies: A potential threat to the premarital screening program. Pak J Med Sci, 35 : 1611.

[18] Naik RP,Haywood C .2015 .Sickle cell trait diagnosis: clinical and social implications. ASH Educ Prog Book, : 160-7.

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