Abstract:
Fasciolosis is a zoonotic helminth infection caused by Fasciola hepatica, the "liver fluke." This parasite infests the liver and bile duct of goats, sheep, cattle, and humans. It is a major neglected tropical disease. The aim of the current study was to evaluate prevalence and molecular characterization of Fasicola hepatica present in small ruminants of district Hangu by ITS1andITS2 genes. In the cross-sectional study, a total of 570 small ruminants were examined for Liver fluke presence in Hangu's local slaughterhouse. Among 570 small ruminants, 325 (male; 187 female; 138) were sheep, and 245 (male; 98 female; 147) were goats. Out of 570 samples, 31 were infected by helminth parasites. Out of 31 infected samples, 18 were sheep and 13 were goats. The prevalence in sheep was 3.15% (male: 1.05%, female: 1.92%), and in goats it was 2.28% (male: 1.40%, female: 0.87%). The genomic DNA was isolated from preserved flukes. The estimation of genetic variation within the species was conducted using conventional polymerase chain reaction (PCR) with a set of arbitrary primers. The amplification of the ribosomal ITS-1 and ITS-2 regions was performed for the isolates. The sequencing of the amplicons was conducted at the ITS-1 and ITS-2 regions. Amplification of both regions was successfully achieved for all samples. The analysis of the acquired ITS sequences in relation to those of established Fasciola species found worldwide and obtained from GenBank demonstrated that the current specimens exhibited complete genetic identity (100%) with F. hepatica.
Page(s):
310-310
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
PCR
,
Fasciolosis
,
sheep
,
Fasciola hepatica
,
Goats