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Tannic Acid Regulates Autophagy in the Ovary of Female Brandt's Voles Afecting their Reproduction
Author(s):
1. Ming-Hao Yu: Department of College of Biological Science and Technology, Yangzhou University,Yangzhou 225009,China
2. Ming-Hui Gu: Department of College of Biological Science and Technology, Yangzhou University,Yangzhou 225009,China
3. Xin Dai: Department of College of Biological Science and Technology, Yangzhou University,Yangzhou 225009,China
4. Dao-Chen Wang: Department of College of Biological Science and Technology, Yangzhou University,Yangzhou 225009,China
5. Sheng-Mei Yang: Department of College of Biological Science and Technology, Yangzhou University,Yangzhou 225009,China
Abstract:
Tannic acid, as a polyphenol, is widely present in dicotyledonous plants and angiosperms. The study found that tannins could afect the ovarian development of Brandt's voles. However, the specific efects of tannic acid on reproduction and its underlying mechanisms are still poorly understood. Therefore, this study explored the efects of tannic acid on the reproduction of Brandt's voles. We treated female Brandt's voles from four weeks postpartum with varying tannic acid doses (0 (control), 0.3% (low dose) or 0.6% (high dose) tannic acids) until 8 weeks (puberty) and 13 weeks (sexual maturity)). Ovarian specimens were collected for immunohistochemical analysis and RT-PCR. The treated Brandt's voles were mated with male Brandt's voles of the same age and we recorded the number of embryos, litters and the male to female ratio of the ofspring. We found that the female Brandt's voles in the low-dose tannic acid group had enhanced reproductive abilities, characterized by reduced follicular atresia and increased numbers of embryos and litters of ofspring. The diferent doses of tannic acid altered the female-male ratio of the Brandt's voles' ofspring. While the low-dose of tannic acid enhanced the level of ovarian autophagy by significantly increasing the expression of Beclin1 and LC3 proteins in the ovaries of the Brandt's voles during sexual maturity. In short, tannic acid can promote the reproduction of Brandt's voles by afecting the level of ovarian autophagy and improving the development of follicles. Tannins can boost the reproduction of Brandt's voles.
Page(s): 51-59
Published: Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 1, Year: 2025
Keywords:
Sex ratio , Reproduction , Autophagy , Tannic acid , Follicle , Ofspring , Brandt vole , Autophagosome
References:
[1] Barra J.M.,Kozlovskaya V.,Kharlampieva E.,Tse H.M. .2020 .Localized immunosuppression with tannic acid encapsulation delays islet allograft and autoimmune-mediated rejection. Diabetes, 69 : 0248-1960.
[2] Chen Z.G.,Luo L.L.,Xu J.J.,Zhuang X.L.,Kong X.X.,Fu Y.C. .2010 .Efects of plant polyphenols on ovarian follicular reserve in aging rats. Biochem. Cell Biol, 88 : 737-745.
[3] Hewison A.J.M.,Gaillard J.M. .1996 .Birth-sex ratios and local resource competition in roe deer, Capreolus capreolus. Behav, 7 : 461-464.
[4] Huang R.,W. R. .2015 .Identifying an essential role of nuclear LC3 for autophagy. Autophagy, 1 : 038016-853.
[5] Kumsta C.,Chang J.T.,Lee R.,Tan E.P.,Yang Y.,Loureiro R.,Choy E.H.,Lim S.H.Y.,Saez I.,Springhorn A.,Hoppe T.,Vilchez D.,Hansen M. .2019 .The autophagy receptor p62/SQST-1 promotes proteostasis and longevity in C. elegans by inducing autophagy. Nat. Commun, 10 : 5648.
[6] Li G.,Yin B.,Wan X.,Wei W.,Wang G.,Krebs C.J.,Zhang Z. .2016 .Successive sheep grazing reduces population density of Brandt's voles in steppe grassland by altering food resources: A large manipulative experiment. Oecologia, 180 : 149-159.
[7] Linklater W.L.,Law P.R.,Gedir J.V.,Preez P. .2017 .Experimental evidence for homeostatic sex allocation after sex-biased reintroductions. Nat. Ecol, 1 : 88.
[8] Liu H.,Wang D.H.,Wang Z.W. .2003 .Energy requirements during reproduction in female brandt's voles (Microtus brandtii). J. Mammal., 84 : 1410-1416.
[9] Luo L.L.,Huang J.,Fu Y.C.,Xu J.J.,Qian Y.S. .2008 .Efects of tea polyphenols on ovarian development in rats. J. Endocrinol. Invest., 31 : 1110-1118.
[10] Moilanen J.,Karonen M.,Tahtinen P.,Jacquet R.,Quideau S.,Salminen J.P. .2016 .Biological activity of ellagitannins: Efects as anti-oxidants, pro-oxidants and metal chelators. Phytochemistry, 02 : 65-72.
[11] Nakashima A.,Tsuda S.,Kusabiraki T.,Aoki A.,Ushijima A.,Shima A.,Sharma S.,Saito S. .2019 .Current understanding of autophagy in pregnancy. Int. J. mol. Sci, 20 : 13.
[12] Pasquariello R.,Verdile N.,Brevini T.A.L.,Gandolfi F.,Boiti C.,Zerani M.,Maranesi M. .2020 .The role of resveratrol in mammalian reproduction. Molecules, 25 : 0003.
[13] Provenza F.D.,Burritt E.A.,Perevolotsky A.,Silanikove N. .2000 .Self-regulation of intake of polyethylene glycol by sheep fed diets varying in tannin concentrations. J. Anim. Sci., 78 : 1206-1212.
[14] Shen M.,Jiang Y.,Guan Z.,Cao Y.,Li L.,Liu H.,Sun S.C. .2017 .Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy. Autophagy, 13 : 1364-1385.
[15] Shi Y.N.,Zhou S.W.,Fan S.F.,Ma Y.H.,Li D.D.,Tao Y.B. .2020 .Encapsulation of bioactive polyphenols by starch and their impacts on gut microbiota. Curr. Opin. Fd. Sci., 11 : 102-111.
[16] Sobhani M.,Farzaei M.H.,Kiani S.,Khodarahmi R. .2020 .Immunomodulatory; anti-inflammatory/ antioxidant efects of polyphenols: A comparative review on the parental compounds and their metabolites. Fd. Rev. Int., : 1-53.
[17] Sun Y.C.,Wang Y.Y.,Sun X.F.,Li S.F.,Zhao L.,Shen Y.,Chen W. .2018 .The role of autophagy during murine primordial follicle assembly. Aging (Albany NY), 10 : 197-211.
[18] Zhou Z.,Lin Q.,Xu X.,Illahi G.S.,Dong C.,Wu X. . .2019b. Maternal high-fat diet impairs follicular development of ofspring through intraovarian kisspeptin/GPR54 system. Reprod. Biol. Endocrinol., 17 : 13.
[19] Zhong W.,Wang G.,Zhou Q.,Wang G.,Environ J. Arid .2006 .Communal food caches and social groups of Brandt's voles in the typical steppes of Inner Mongolia, China. , 06 : 398-407.
[20] Zhihan T.,Xinyi M.,Qingying L.,Rufei G.,Yan Z.,Xuemei C.,Yanqing G.,Yingxiong W.,Junlin W. .2019 .Autophagy participates in cyst breakdown and primordial folliculogenesis by reducing reactive oxygen species levels in perinatal mouse ovaries. J. Cell Physiol, 234 : 6125-6135.
[21] Yin B.,Li G.,Wan X.,Shang G.,Wei W.,Zhang Z. .2017 .Large manipulative experiments reveal complex efects of food supplementation on population dynamics of Brandt's voles. , 60 : 911-920.
[22] Yim W.W.,Mizushima N. .2020 .Lysosome biology in autophagy. Cell Discov, 6 : .
[23] Yang S.,Shao S.,Huang B.,Yang D.,Zeng L.,Gan Y.,Long D.,Chen J.,Wang J. .2020 .Tea polyphenols alleviate tri-ortho-cresyl phosphateinduced autophagy of mouse ovarian granulosa cells. Environ. Toxicol., 35 : 478-486.
[24] Yadav P.K.,Tiwari M.,Gupta A.,Sharma A.,Prasad S.,Pandey A.N.,Chaube S.K. .2018 .Germ cell depletion from mammalian ovary: Possible involvement of apoptosis and autophagy. J. biomed. Sci., 25 : 36.
[25] Xie X.,Yi W.,Wu P.,Yan N.,Yang Q.,Tian H.,Xiang C.,Du S.,Getachew M.,Zuo A.E.,Ying X.,C. X. .2017 .Green tea polyphenols, mimicking the efects of dietary restriction, ameliorate high-fat diet-induced kidney injury via regulating autophagy flux. Nutrients, 9 : 14.
[26] Wishart A.E.,Williams C.T.,McAdam A.G.,Boutin S.,Dantzer B.,Humphries M.M.,Coltman D.W.,Lane J.E. .2018 .Is biasing ofspring sex ratio adaptive? A test of Fisher's principle across multiple generations of a wild mammal in a lfuctuating environment. Proc. R. Soc. B Biol. Sci., 285 : .
[27] Weng X.C.,Ma X.S.,Wang Q.Z.,Xu K.L.,Hu X.S.,Liu W.B.,Zhang C. .2019 .Efect of hypothyroidism on CYP51 and FSHR expression in rat ovary. Theriogenology, 07 : 145-151.
[28] Weldy M.J.,Epps C.W.,Lesmeister D.B.,Manning T.,Linnell M.A.,Forsman E.D. .2019 .Abundance and ecological associations of small mammals. J. Wildl. Manage., 83 : 902-915.
[29] Watanabe R.,Kimura N. .2018 .Non-suckling starvation of neonatal mice promotes primordial follicle formation with activation of ovarian autophagy. J. Reprod. Dev., 64 : 126-94.
[30] Verheyden-Tixier H.,Duncan P. .2000 .Selection for small amounts of hydrolysable tannins by a concentrate-selecting mammalian herbivore. J. chem. Ecol., 26 : 351-358.
[31] Tang Z.,Xu R.,Shi Z.,Zhang C.,Yang Y.,Lin H.,Liu Q.,Lin Y.,Geng F.,Wang B.,Z. B. .2021 .HIF-1alpha protects granulosa cells from hypoxia-induced apoptosis during follicular development by inducing autophagy. Front. Cell Dev, 9 : 631016.
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