Abstract:
Hypocotyls of Papaver somniferum exhibited growth only when inoculated on 1/2 x MS medium. callus induction occurred on the addition of auxin and a kinetin. Best callus resulted at 0.5 mg/L of 2, 4-D and 1.0 mg/L of K in presence of coconut milk. Early browning and death of tissue was controlled by the addition of sodium diethyl dithiocarbamate at 250 mg/L. However, the addition of this substance necessitated a change in the hormone concentration and an excellent callus resulted at 0.1 mg/L of 2, 4-D, kinetin 2.0, SDD 250 mg/L and CM at 5% v/v). The cultures remained alive for a longer duration on this combination and could be easily subcultured.
Page(s):
13-18
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Botany, Volume: 15, Issue: 1, Year: 1983