Time Kill Assay and Bactericidal Mechanism of Action of Ethanolic Flowers Extract of Sphaeranthus indicus | [RADS Journal of Pharmacy & Pharmaceutical Sciences • 2019]

Author(s):

1. Nazish Mumtaz: Department of Pharmaceutics, Faculty of Pharmacy, Benazir Bhutto Shaheed University, Lyari, Karachi, Pakistan

2. Muhammad Arif Asghar: Department of Pharmaceutics, Faculty of Pharmacy, Jinnah Sindh Medical University, Rafiqui H.J. Shaheed Road, Karachi, Pakistan

3. Syed Baqir Shyum Naqvi: Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Karachi, Sharea Madinat Al-Hikmah, Muhammad Bin Qasim Avenue, Karachi, Pakistan

4. Muhammad Asif Asghar: Food and Feed Safety Laboratory, Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Shahrah-e-Salimuzzaman Siddiqui, Off University Road, Karachi, Pakistan

5. Muhammad Liaquat Raza: Department of Clinical Sciences, Biomedical Centre – BMC, Lund University Hospital, Lund, Sweden

6. Ahad Abdul Rehman: Department of Pharmacology, Faculty of Pharmacy, Jinnah Sindh Medical University, Rafiqui H.J. Shaheed Road, Karachi, Pakistan

Abstract:

Objective: The purpose of the study was to identify the bactericidal mechanism of action and time kill assay of a medicinal plant Sphaeranthus indicus Linn against some highly resistant Gram positive and Gram negative clinical pathogens. Methods: Antimicrobial action of this plant was compared with four different antibiotics using disk diffusion method. The MIC and MBC of the extract were measured by broth dilution method. The bacterial killing mechanism of extract was examined using SEM technique. Results: It was observed that the zones of inhibition produced by the plant extract were higher than that of some well-reputed antibiotics. The values of MIC and MBC indicated that the extract had strong inhibitory and bactericidal activity against tested isolates. The plant extract achieved a more drop in growth profile of S. pneumonia, S. typhi, E. coli, and P. aeruginosa within 20 mins. After 12 h exposure with extract, the SEM images of S. pneumonia showed the destruction of cell membrane and cells were completely lysed. Conclusion: It is concluded that ethanolic flowers extract of S. indicus has promising bactericidal potential at very low concentration. Its time killing was extremely faster especially against S. pneumonia. Its flowers extract produced bactericidal action through a cell membrane disruption of bacteria.

Page(s): 27-33

DOI: DOI not available

Published: Journal: RADS Journal of Pharmacy & Pharmaceutical Sciences, Volume: 7, Issue: 1, Year: 2019

Keywords:

Antimicrobial activity , mechanism indicus , Streptococcus pneumonia , time kill assay

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