Abstract:
The eco-friendly synthesis of nanoparticles has gained attention due to their diverse biological applications. This study presents the sustainable production of silver (Ag) and nickel oxide (NiO) nanoparticles (NPs) using water-soluble polysaccharides extracted from chia (Salvia hispanica) seed mucilage. Polysaccharides were extracted via ethanol precipitation, and the bio synthesized NPs were characterized by UV-visible spectroscopy, revealing absorption peak sat 473 nm for Ag-NPs and 343 nm for NiO-NPs. The antioxidant potential of the NPs was assessed using the DPPH assay, with Ag-NPs and NiO-NPs showingEC50 values of 118.85 ± 2.52 µg/mL and 97.21 ± 1.39 µg/mL, respectively, compared to 80.78 ± 1.65 µg/mL for ascorbic acid. Additionally, both NPs demonstrated promising in vitro anti diabetic activity, with IC50 values of 87.70± 3.65 µg/mL (Ag-NPs) and 146.15 ± 1.94 µg/mL (NiO-NPs) against alpha amylase, compared to a carbose (71.75 ± 2.63 µg/mL). The antibacterial effectiveness of the nanoparticles against three distinct variants of human pathogens was assessed using the agar well diffusion technique. The findings indicated that Ag-NPs effectively suppressed the growth of three pathogenic strains: Staphylococcus aureus (9.5mm ± 0.5mm), Escherichia coli (11.75mm ±0.82mm), and Hemophilus influenza (8.5mm ± 0.82mm). These findings indicate that chia seed-derived polysaccharides are effective in synthesizing bioactive Ag-NPs and NiO-NPs with strong antimicrobial and antioxidant properties, making them valuable for biomedical applications. This study underscores the potential of mucilage polysaccharides as a sustainable resource for producing bioactive nanoparticles.
Page(s):
125-125
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
antimicrobial
,
antioxidant
,
Nanoparticles
,
Chia
,
Polysaccharides