Anti-diabetic and Neuroprotective Effects of Transformed Ajuga bracteosa Hairy Roots Regenerants | [Abstract Book on Global Science Technology and Management Conference • 2023]

Author(s):

1. Samina Rubnawaz: Department of Biochemistry, Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, Pakistan, Faculty of Biological Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

2. Bushra Mirza: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Lahore, College for Women University, Lahore, Pakistan

Abstract:

Diabetes Mellitus (DM) is among major risk factors for Alzheimer’s Disease (AD) in aged people and development of potential agents for dual therapy of DM and AD has received much attention. Ajuga bracteosa has extensively been used in folk medicine for many diseases. Therefore, present study aimed to investigate A. bracteosa for the treatment of DM and AD. Extracts from regenerants of transgenic hairy roots and wild plants were evaluated for a-amylase, ß-glucosidase, acetylcholinesterase and butyrylcholinesterase enzymes inhibition. Molecular docking was carried out to find the possible interactions of these enzymes with selected phytoecdysteroids using AutoDockTools (ADT) software. Further in-vivo studies were performed by inducing symptoms of DM and AD in BALB/c mice. Levels of blood glucose and brain neurotransmitters were monitored along with behavioral changes, biochemical and histochemical assessment. Plant extracts showed enzymes inhibition in concentration dependent manner. Transgenic extracts were found more potent inhibitors compared to wild plants. Molecular docking of the compounds isolated from A. bracteosa revealed their activity against these 4 enzymes. Plant extracts significantly reduced blood glucose level in a time dependent manner and elevated liver function markers were reinstated. Transgenic extracts raised the level of dopamine and serotonin in brain significantly when compared to Alzheimer control group. Moreover, a decrease in memory impairment was found in the mice groups treated with plant extracts as compared to control. These results indicate the potential of Ajuga bracteosa as multifunctional remedy for the dual therapy of DM and AD and demand further investigation.

Page(s): 63-63

DOI: DOI not available

Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023

Keywords:

Diabetes Mellitus , Alzheimers Disease , Ajuga bracteosa , Neuroprotective Effects

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