Abstract:
The current work report that the synthesis of iron dopped hydroxyapatite nanocomposite was done by slat melting method. The characterization of the prepared nanocomposite was done by FTIR, XRD, SEM, and EDX which confirms the desired synthesis. FTIR analysis affirms functional group, XRD analysis show rhombohedral phase of the prepared nanocomposite and non uniform nature can be seen through SEM image. EDX analysis showed the doping of iron oxide nanoparticle with hydroxyapatite. A new colorimetric biosensor was developed for ascorbic acid detection based on low-cost 3, 3', 5, 5' Tetramethylbenzidine (TMB) which act as an oxidative chromogenic substrate which reacts with hydrogen peroxide in the presence of iron dopped hydroxyapatite nanocomposite. This results in the formation of colorless product that can be visualized with naked eyes and confirmed by UV-Vis spectrophotometer. The optimization of various parameters (time, pH, concentrations, loading of iron dopped hydroxyapatite nanocomposite) was done to obtain best response of the proposed sensor. This sensor showed a wide linear range of 1x10-9-3.2 x10-7 M and low limit of detection 1.07x10-8 M. The selectivity of the proposed sensor was compared with the potential interfering species. The incubation time was estimated to be just 5 minutes for the proposed sensor. Additionally, the sensor was effectively applied to test ascorbic acid in the urine samples of diabetes mellitus patient.
Page(s):
419-419
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
hydrogen peroxide
,
ascorbic acid
,
peroxidase mimic
,
Colorimetric sensor
,
iron dopped hydroxyapatite nanocomposite