Topical Analgesic and Anti-Inflammatory Properties of Bioengineered Juglans regia L. Silver Nanoparticles | [Pakistan Journal of Biological Sciences • 2023]

Author(s):

1. Areej Awadallah: Department of Pharmaceutics, Faculty of Pharmacy, Mutah University, Alkarak, Jordan

2. Afaf Hasan Al-Nadaf: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Alkarak, Jordan

Abstract:

Background and Objective: Research has demonstrated the antibacterial, anti-angiogenetic, antiviral, anti-inflammatory, anticancerand antioxidant properties of colloidalsilver dueto its biological, optical and electrical properties. Theaimof this study was the anti-inflammatory effect of the silver bioengineered nanoparticles by using the acetonitrile-unripe fruit extract of Juglans regia L., on experimental animal model. Materials and Methods: The study uses various techniques to characterize nanoparticles, including ultraviolet spectra, dynamic light scattering and Fourier transform infrared. The study used carrageenan-induced rat paw edema as an induction model for inflammation and assessed its antinociceptive effects in mice using the formalin test. As well as evaluation of proinflammatory cytokines|L-6, TNFand IL-1. ResultsT:he produced AgNPs were more compact and stable, according to physical characterization methods compared to chemical prepared nanoparticles. The formulation combining unripe fruit bio-reduced nanoparticles and extract, UF, shows a greater acute anti-inflammatory effect, while leaf extract has a better late anti-inflammatory effect. These bioengineered nanoparticles show efficient /in vivo anti-acute inflammation, reducing skin inflammation through decreased cellular infiltrates and cytokine release. Conclusion: Juglans regia L., extract and silver nanoparticles show notable effects in both the early and late stages of the antinociceptive formalin test. While, bioengineered NP/UF and NP/LV can be used as topical analgesics. The potent topical anti-inflammatory and analgesic effects of these medications provide a sufficient basis for the use of this plant material in dermatological products.

Page(s): 493-503

DOI: 10.3923/pjbs.2023.493.503

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 26, Issue: 9, Year: 2023

Keywords:

Juglans regia , Silver , Juglans regia , Juglans regia , metal nanoparticles , pellcle , Bioengineering , antinflammatory

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