Abstract:
Diabetes mellitus is a heterogeneous metabolic disorder causing several health complications and impose economic burden which apparently effects human life. Polysaccharides have been studied for its promising role to reduce blood glucose level, converse insulin resistance and prevent diabetes complications. The current study was performed to evaluate the invitro and invivo antidiabetic potential of polysaccharides extracted from Lepidium sativum (Cress) seed mucilage. Polysaccharides from cress seed mucilage were precipitated by 75% ethanol and were freeze dried. Freeze-dried EPP were further fractioned through size exclusion chromatography with column loaded with Bio Gel P10. The monosaccharides compositions of fraction confirms the presence of saturated and unsaturated uronic acid, pentose and hexose content. Fractionshaving notable monosaccharides significant were processed for evaluation of their invitro and invivo antidiabetic potential. Among all fractions, F53exhibited significant inhibition of a-amylase having EC50 value of 190.96 µg/mL, and a-glucosidase (EC50 value = 146.28 µg/mL) and promoted yeast glucose uptake ability by 93.67%. The administration of F53 polysaccharides in alloxanised mice decrease blood glucose level by 91.50% compared to glibenclamide (93.85%) which was implied as reference drug after 14 days of treatment. Similarly, compared to diabetic control group, significant weight gain was regarded for alloxanised mice treated with F53, F18 and F52 polysaccharides fractions. Furthermore F53 and F18 fractions also restored the lipid profile by reducing blood serum triglycerides (TG), low density lipid (LDL), total cholesterol (TC) and enhancinghigh density lipid (HDL), content. Collectively, the finding of this study demonstrated that the antidiabetic effect of cress seed mucilage polysaccharides may be associated with regulation of diabetes and can be incorporated in health care foods, drugs to control diabetes.
Page(s):
99-99
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Diabetes mellitus
,
Diabetes mellitus
,
Polysaccharides
,
Lepidium sativum
,
Mucilage