Abstract:
Marine algae are a potential bioactive source that began to be developed as a new pharmaceutical agent, including antidiabetic. The objective of this research was to determine the potential of polyphenols and phlorotannins extract from Sargassum hystrix (J. Agardh, 1847) and Eucheuma denticulatum [(N. L. Burman) F. S. Collins & Hervey, 1917] in inhibiting a-amylase and a-glucosidase. Polyphenols were extracted using 50 % methanol, and phlorotannins were extracted using methanol, and the non-lipid layer was separated by using distilled water, methanol and chloroform, and then partitioned using ethyl acetate twice. The total content of polyphenols and phlorotannins were analyzed. Both types of the compounds were tested to determine their ability to inhibit a-amylase and a-glucosidase activity. Total phenols content of S. hystrix and E. denticulatum were observed to be 3.17 g GAE. 100 g-1 extract and 0.33 g GAE. 100 g-1 extract, respectively. Total phlorotannin content of S. hystrix and E. denticulatum were obtained 0.02 g PGE. 100 g-1 extract and 0.02 g PGE. 100 g-1 extract, respectively. The results showed that polyphenols S. hystrix (IC50 = 0.58±0.01 mg.mL-1) can inhibit a-amylase, similar to acarbose (IC50 = 0.53±0.00 mg.mL-1) and phloroglucinol (IC50 = 0.56±0.01 mg·mL-1), but inhibiton activity of polyphenol and phlorotannin from E. denticulatum was lower (IC50 = 1.43±0.19 and 1.92±0.14 mg.mL-1, respectively). Inhibitory activity of polyphenols from S. hystrix (IC50 = 0.59±0.02 mg.mL-1) in inhibiting a-glucosidase was also similar to acarbose (IC50 = 0.61±0.01 mg.mL-1) and phloroglucinol (IC50 = 0.56±0.05 mg.mL-1), but inhibiton activity of polyphenol and phlorotannin from E. denticulatum was also lower (IC50 = 1.43±0.19 and 0.86±0.06 mg. mL-1, respectively). So, S. hystrix had more potential as an antidiabetic substance compared to E. denticulatum.
Page(s):
1-8
DOI:
DOI not available
Published:
Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 55, Issue: 3, Year: 2018
Keywords:
aamylase
,
antidiabetic activity
,
Sargassum hystrix
,
aglucosidase
,
Eucheuma denticulatum