The phenotypic polymorphism of saliva from dental caries patients | [BioSight • 2023]

Author(s):

1. Zaid Majeed Khan: Dr. Zafar H Zaidi Center for Proteomics, University of Karachi,Karachi 75270,Pakistan

2. Humera Waheed: Dow College of Biotechnology, Dow University of Health Sciences,Karachi 75270,Pakistan

3. Zohaib Khurshid: Department of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University,Al Ahsa 31982,Saudi Arabia

4. Syed Faraz Moin: Dr. Zafar H Zaidi Center for Proteomics, University of Karachi,Karachi 75270,Pakistan

Abstract:

Introduction: The tooth decay or dental caries is a disease that results in imbalance in mineralization process of the tooth, cavitation and sensitivity as well as pulp infection that leading to inflammation and eventually tooth loose. Saliva which is in direct contact with the dentine, can serve as a medium for analysis of components or factors that show the difference in normal healthy and disease conditions. Methodology: Here, a biochemical analysis of individual saliva samples from healthy control and caries patients were carried out to see the differences in protein profiles. The unstimulated saliva samples were analyzed through sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE), two-dimensional gel electrophoresis (2D gel), zymography and secretary IgA (sIgA) antibody ELISA procedure. Results: Differences were observed in individual samples not only in polymorphic band pattern on SDS-PAGE gels but also in activity of proteases in the saliva and the concentration of sIgA antibody. Conclusion: These were the initial results obtained from the study that was further optimized and evaluated with accuracy and reported elsewhere with statistical figures.

Page(s): 33-40

DOI: 10.46568/bios.v4i1.76

Published: Journal: BioSight, Volume: 4, Issue: 1, Year: 2023

Keywords:

SDSPAGE , Saliva , Elisa , caries , sIgA , Zymography

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