The combination of crocin with cisplatin suppresses growth of gastric carcinoma cell line BGC-823 and promotes cell apoptosis | [Pakistan Journal of Pharmaceutical Sciences • 2017]

Author(s):

1. Yushuang Luo: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine, Xining, China; Department of Oncology, Affiliated Hospital of Qinghai University, Kunlong Road 16, Tongren Road 29, Xining, China

2. Sen Cui: Hematology Department, Affiliated Hospital of Qinghai University,Xining,China

3. Feng Tang: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine,Xining,China

4. Cunfang Shen: Department of Oncology, Affiliated Hospital of Qinghai University,Kunlong Road 16, Tongren Road 29, Xining,China

5. Yujuan Qi: Department of Oncology, Affiliated Hospital of Qinghai University,Kunlong Road 16, Tongren Road 29, Xining,China

6. Dianxiang Lu: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine,Xining,China

7. Lan Ma: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine,Xining,China

8. Yingzhong Yang: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine,Xining,China

9. Yan Li: Department of Oncology, Affiliated Hospital of Qinghai University,Kunlong Road 16, Tongren Road 29, Xining,China

10. Rong Chen: Department of Oncology, Affiliated Hospital of Qinghai University,Kunlong Road 16, Tongren Road 29, Xining,China

11. GE Ri-li: Research Center for High Altitude Medicine, Qinghai University, Medical School of Medicine,Xining,China

Abstract:

This study aimed to investigate the efficacy of crocin alone and in combination with cisplatin in the therapy of gastric carcinoma cells. In this study, human gastric carcinoma cell line BGC-823 was purchased and maintained in standard condition. Crocin, cisplatin and crocin plus cisplatin diluted to different concentrations were added into medium, respectively. MTT assay and flow cytometry were performed to test the anti-proliferation effects and apoptosis rates of cells, respectively. In addition, quantitative RT-PCR was used to detect the mRNA expression of apoptosisrelated genes, such as p53, Bax and Bcl-2. After treated with different concentrations of crocin, the inhibition ratio and apoptosis rate of BGC-823 cells were not significantly changed. However, the tumor cell inhibition ratio and apoptosis rate in crocin plus cisplatin group were significantly higher than that in cisplatin, crocin and control group (p<0.05). The treatment of crocin plus cisplatin significantly increased the expression of p53 and Bax (p< 0.05), and significantly decreased the Bcl-2 expression (p<0.05). Collectively, our data demonstrated for the first time that crocin plus cisplatin may be used as a new anticancer drug for the treatment of gastric cancer.

Page(s): 1629-1634

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 30, Issue: 5, Year: 2017

Keywords:

gastric cancer , Cisplatin , Apoptosis , cell proliferation , crocin

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