Abstract:
Nucleotide-binding site (NBS) domain genes are one of a major superfamily of resistance genes, which are implicated in plant immune systems. The current study identified a total of 12,820 genes in land plants covering from mosses to angiosperms. Interestingly, we did not find any NBS genes in some lower ancient plants, known as the least common ancestor of land plants. The number of NBS copies varied from species to species corresponding with plant genome size. The classification of NBS genes identified 168 classes with several novel domain architectures patterns encompassing significant diversity among plant species. Several classical and species-specific structural patterns (TIR-NBSTIR-Cupin, TIR-NBS-Prenyltransf, etc.) were discovered. The orthologs were grouped into 603 Orthogroups (OGs) covering more than 98% of the total NBS genes, demonstrating core (OG0, OG1, etc.) and species-specific groups (OG80, OG85, etc). The expression profiling presented the putative upregulation of OG2, OG6, and OG15 in different tissues under various biotic and abiotic stresses among Arabidopsis, maize, and cotton (susceptible and tolerant lines). The genetic variation between susceptible (Coker 312) and tolerant (Mac7) G. hirsutum accessions identified several variants at different impact levels e.g., 6,583 and 5,173 variants in Mac7 and Coker 312, respectively. The proteinligand and proteins-protein interaction showed a strong interaction of some putative NBS proteins with ADP/ATP and different core proteins of begomovirus. The functional validation of GaNBS (OG2) in naturally resistant cotton through virus-induced gene silencing (VIGS) demonstrated its putative role in CLCuD resistance. Enhanced virus titer was witnessed in G2 silenced plants both under viruliferous whitefly exposed and graft inoculated G. arboretum plants as compared TRV:00 inoculated control plants. The results presented in this study will not only provide a reference study for a deep evolutionary study of superfamily genes but also provided genetic markers, which will be helpful for marker-assisted breeding.
Page(s):
195-195
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Classification
,
Diversity
,
Genomewide
,
VIGS
,
NLR
,
evolution
,
expression
,
Land Plants