Author(s):
1. Saher Riaz:
Department of Biochemistry, IBBB The Islamia University of Bahawalpur, Bahawalpur, Pakistan
2. Mirza Imran Shahzad:
Department of Biochemistry, IBBB The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
TB is still a serious public health concern across the world. M. tb is thought to infect one-third of the world’s population. There are some obstacles in the protective response of BCG vaccine. The effectiveness of BCG against TB varies by population. Novel DNA vaccine is needed to maintain ongoing TB situations. DNA vaccine has proven to be one of the most promising techniques for the protection against TB. DNA vaccine is effective against TB as it initiates cellular immune response. Current study is designed to develop M. tb specific Rv1926c DNA vaccine expressing Mpt63 immunogenic protein. In this research, the protective effectiveness of a DNA vaccination expressing Mpt63 was investigated in a mouse model. Gene Rv1926c after amplification was restricted digested to be directly cloned into mammalian expression vector. Sanger sequencing was used to validate the existence of insert in the isolated plasmid. Endotoxin free DNA prep was made and plasmid was administered to mice intramuscularly and intradermally. Mpt63 based DNA vaccine was tested alone and in combination with BCG. Vaccine immune response was evaluated through different techniques and antibody titer was checked through multiplex microbead assay.
Page(s):
78-78
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
DNA vaccine
,
multiplex microbead assay
,
Mpt63Rv1926c
References:
References are not available for this document.
Citations
Citations are not available for this document.