THE PERENNIAL SWEET SORGHUM CULTIVAR AND ITS RECRUITING RHIZOSPHERE DOMINANT BACTERIAL TAXA UNDER FIELD GROWTH | [Pakistan Journal of Botany • 2022]

Author(s):

1. GUI HUA LU: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences,Huaiyin Normal University, Huai’an, China;Yunnan Eco-Agriculture Research Institute, Kunming, China

2. ZHIYE NA: Yunnan Eco-Agriculture Research Institute,Kunming,China

3. ZIJIAN ZHANG: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an,China

4. RUI CAO: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an,China

5. ALIYA FAZAL: Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing ,China

6. SHOUCHENG HUANG: College of Life and Health Science, Anhui Science and Technology University,Fengyang ,China

7. HANYUE ZHANG: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an,China

8. JIALING CHEN: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an 223300,China

9. RUI CHENG: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an 223300,China

10. YAN LI: Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University,Huai'an 223300,China

11. YONGHUA YANG: Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210023,China

12. HONGJUN YANG: Yunnan Eco-Agriculture Research Institute,Kunming 650106,China

13. ZHONG YUAN NA: Yunnan Eco-Agriculture Research Institute,Kunming 650106,China

Abstract:

Breeding new perennial sorghum germplasms has been ongoing for decades since perennial crops provide significantly greater environmental benefits in terms of resource utilization, soil erosion, and soil conservation. However, perennial sweet sorghum cultivars or varieties have rarely been reported to date. Additionally, little is known about the potential consequences of perennial sorghum on belowground soil microbial communities. In this study, we reported the perennial sweet sorghum cultivar NaPBS778 (simply N778) with prominent panicle length, plant height, stalk weight, and Brix, which was generated from an annual sweet sorghum cultivar via the Gene Phenotype Induction Technique (GPIT). Further, we designed an experiment to detect the dominant rhizosphere bacterial taxa of the perennial sweet sorghum cultivar N778 and its control sorghum line TP60 at the flowering and maturation stages under natural field growth conditions. The Illumina MiSeq platform was utilized to high-throughput sequence amplicons of the V3-V4 hypervariable region of the 16S rRNA gene (16S rDNA). According to our findings, almost all six alpha diversity indices of N778 rhizosphere soil from either aerial roots and primary roots showed no significant difference when compared to those of TP60 at the same growth stage. There were also no significant differences in the beta diversity of rhizosphere soil between N778 and its control TP60 at the flowering stage. at the maturation stage; however, significant differences in taxonomic and phylogenetic beta diversity of rhizosphere soil were found between N778 and its control TP60. Furthermore, at the maturation stage, the relative abundances of two genera, Pseudarthrobacter and Pseudomonas, were significantly higher in the rhizosphere soil of N778 than in all other groups. Our results indicate that two dominant OTUs belonging to Pseudarthrobacter and Pseudomonas with stress tolerant phenotypes are significantly enriched in the rhizosphere of N778 at the maturation stage, implying that the perennial sweet sorghum cultivar N778 could recruit potential psychrotolerant bacterial taxa into its rhizosphere and assist it in surviving the winter below freezing temperature.

Page(s): 865-878

DOI: 10.30848/PJB2022-3(46)

Published: Journal: Pakistan Journal of Botany, Volume: 54, Issue: 3, Year: 2022

Keywords:

highthroughput sequencing , Sorghum bicolor L , Perennial sweet sorghum cultivar , 16S rRNA gene 16S rDNA , Illumina MiSeq , Rhizosphere bacteriome , Dominant taxa

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