A Comparison of Thioredoxin 1 Gene Expression in Schizophrenia Patients before and after Treatment with Risperidone | [Pakistan Journal of Medical and Health Sciences • 2021]

Author(s):

1. ESFANDIAR AZIZI: Department of Immunology, School of Medicine, Ilam University of Medical Sciences,Ilam,Iran

2. MORTEZA HOSSEINZADEH: Department of Immunology, School of Medicine, Ilam University of Medical Sciences,Ilam,Iran

3. PARVIZ VAHDATIAN: Department of Immunology, School of Medicine, Ilam University of Medical Sciences,Ilam,Iran

4. AMIR ADIBI: Department of Child and Adolescent Psychiatry, School of Medicine, Ilam University of Medical Sciences,Ilam,Iran

5. AKBAR AZIZIFAR: Department of Psycholinguistics, School of Medicine, Ilam University of Medical Sciences,Ilam,Iran

6. HOJAT SAYYADI: Department of Biostatistics, School of Health, Ilam University of Medical Sciences,Ilam,Iran

7. OMRAN DAVARINEJAD: Department of Psychiatry, School of Medicine, Kermanshah University of Medical Sciences,Kermanshah,Iran

8. HOSSEIN SEIDKHANI: Department of Biostatistics, School of Health, Ilam University of Medical Sciences,Ilam,Iran

9. KARIMEH HAGHANI: Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences,E-mail address:,Iran.

Abstract:

Background: Schizophrenia is a mental disorder characterized by distortions in thinking, perception, emotions, language, self-sense, and behavior. Recent research suggests that Reactive Oxygen Species (ROS) are involved in the pathophysiology of schizophrenia. Studies have also shown the increased plasma and serum levels of the Trx1 molecule in schizophrenia patients. In the present study, the researchers compared the expression levels of Trx1 mRNA in peripheral blood leukocytes of Iranian schizophrenia patients compared to healthy controls. Methods: First-episode patients (n=35) who met DSM-IV criteria for schizophrenia were recruited from patients referred to psychiatrists in the city of Ilam and Farabi Hospital in Kermanshah. Healthy people were also selected by recruiting people who, according to a psychiatrist, did not have any mental illness. Diagnoses were made for each patient by two independent experienced psychiatrists and confirmed by the Structured Clinical Interview for DSM-IV (SCID). Patients were treated with risperidone for three months and then compared with thirty- five healthy volunteers. Patients were sampled before and after treatment and then by RNA Extraction and DNA synthesis, Trx1 gene expression was performed by real-time PCR method. Results: Comparison of Trx1 gene expression in PBMCs of schizophrenic patients before and after treatment with the control group showed that the expression of Trx1 gene of the “before” treatment group was significantly increased compared with that of the control group (P= 0.0007). Also, Trx1 gene expression in PBMCs of “before” and “after “groups showed that Trx1 gene expression of “after” group was significantly decreased compared to the “before” group (P= 0.014). These results showed that the mean of positive, negative, and general psychopathology was reduced significantly in schizophrenic patients before and after treatment in all three cases (P <0.001). Conclusion: the expression of TRX in PBMCs of schizophrenic patients decreased after risperidone treatment. This reduction of expression was statistically significant and indicates the possible effect of risperidone on the expression of the TRX gene in PBMCs of these patients and decreased gene expression is associated with reduced symptoms. Confirmation of the achievement of this study requires further research.

Page(s): 1551-1556

DOI: 10.53350/pjmhs211551551

Published: Journal: Pakistan Journal of Medical and Health Sciences, Volume: 15, Issue: 5, Year: 2021

Keywords:

Schizophrenia , Risperidone , Thioredoxin

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