Abstract:
Colchicine induced tetraploids were obtained by the application of aqueous solutions of colchicine with five different concentrations (.02, 04,. 06,. 08, and . 10%) at five different timings (8, 12, 16, 20 and 24 hours) both by seeding and seed treatment of Trifolium alexandrianum L. (Berseem), a commonly used fodder crop throughout Pakistan. Cytomorphological characters of diploid and induced tetraploids revealed that the seed treatment with 04 percent colchicine solution for 24 hrs. and the seeding treatment with an aqueous solution of the same concentration for 4 hrs. were most satisfactory from stand point of seeding survival and incidence of tetraploidy. Tetraploids exhibited giganticism in all the cytomorphological traits of a plant. Tetraploids obtained through seed treatment showed better performance than those obtained through seeding treatment. Appreciable increase in grain weight and pollen size was also recorded. It is concluded that the tetraploids produced can successfully be exploited in practical breeding.
Page(s):
305-309
DOI:
DOI not available
Published:
Journal: Biologia, Volume: 33, Issue: 2, Year: 1987