Abstract:
Roadside plants are consistently exposed to dust. Automobiles release a number of toxic metals into the surrounding environment. Deposition of dust on roadside plant and its impact on leaf epidermal traits. Present study evaluated the effect of pollution on different tissues and cells of epidermal, mechanical, storage, vascular bundles (protoxylem, metaxylem and phloem) leaf blade thickness, cortical cells, trichomes and sclerenchyma thickness and stomata of ten different plant species. Leaf samples of plant species (Cassia fistula L. Punica grantum L., Dalbergia sissoo (Roxb), Euclyptus obliqua L. Her., Polyalthia longifolia (Sonn), Terminalia arjuna (Roxb), Callistemon viminalis R. Br., Jatropha integerrima L., Heplophragma adenophyllum (Wall.) and Jasminum sambac L.) growing along roads i.e. College Road Township. Lahore, Punjab, Pakistan, were collected. In this project for microstructural studies free hand sectioning technique was used and the material was fixed in formalin acetic alcohol (v/v formaline 10%, acetic acid 5%, ethyl alcohol 50% and distilled water 35%). Permanent sectioning slides were prepared by serial dehydrations 30%, 50%, 70%, 90% and 100% in ethanol using standard double-stained technique of Safranine and Fast green stains. Extensive variations were observed in all collected leaves, as epidermal cell area reduced in C. fistula and D. sissoo. Leaf bade thickness was maximum in E. obliqua and reducd in Terminalia arjuna. The leaf area showed much distinct among species, reduced vascular bundles area (protoxylem, metaxylem and phloem) in C. viminalis and D. sissoo. The stomatal density was maximum in J. integerrima and reduced in J. sambac. The sclerenchyma thickness of leaf was higher in D. sissoo and is less in C. fistula. This is due to the fact of adaptations in such environmental conditions that polluted road by heavy vehicles. Some additional studies mainly in experimental conditions are required in order to obtain more exactly data regarding these microstructural variations and need to compare different roads.
Page(s):
177-177
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
stomatal density
,
Trichome
,
sclerenchyma
,
Microstructures