TFT1 and TFT10 most likely interact with sucrose-phosphate synthase (SPS) in tomato. | [International Journal of Agriculture and Biology • 2012]

Author(s):

1. Zhi Hai Yu: Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China

2. Na Cui: Key Laboratory of Protected Horticulture of Liaoning Province, Shenyang 110866, China

3. Xue Fei Dong: Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China

4. Ming Li Han: Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China

5. Quan Sun: Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China

6. Li Ping Bai: Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China

7. Tian Lai Li: Key Laboratory of Protected Horticulture of Liaoning Province, Shenyang 110866, China

Abstract:

SPS is a target for 14-3-3s and there are 12 isoforms (TFT1-TFT12) of 14-3-3s in tomato (Solanum lycopersicum). Bornke’s research showed that T14-3d and T14-3g, isoforms of 14-3-3s, can interact with SPSA in tobacco (Nicotiana tabacum). Analyzing the physical and chemical properties of 14-3-3s, 3-D structures and motifs of tomato 14-3-3s and SPS with bioinformatics softwares, we predicted, which isoform(s) has (have) the ability to bind with SPS based on Bornke’s research. The results showed that aliphatic indexes are all greater than 70 and the grand average of hydrophobicity ranged from -0.701 to -0.269 of 14-3-3s in tomato, indicating that tomato 14-3-3s are hydrophilic proteins with high thermal stability. The similarities between TFT1 and T14-3d, TFT10 and T14-3 g had 95% and 94% identity, respectively. By constructing a phylogenetic tree, TFT1\\T14-3d\\TFT10\\T14-3 g are found to be on the same branch. This indicated that tft1 and t14-3d, tft10 and t14-3 g are 2 groups of orthologous genes. The 3-D structure modeling showed that TFT1 and T14-3d, TFT10 and T14-3 g have similar structures. Likewise, structures of tomato SPS and tobacco SPSA were very similar. Moreover, all tomato 14-3- 3s had an auto-inhibitory domain in C-terminal variable region to prevent the interaction between 14-3-3s and SPS with the exception of TFT1 and TFT10. From these comprehensive analyses, we clearly deduced that TFT1 and TFT10 are those most likely to interact with SPS in tomato.

Page(s): 81-86

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 14, Issue: 1, Year: 2012

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