The Synergistic Effect of Gw9508 and S14161 Small Molecules on Apoptotic Gene Expression and Viability on A549 Cell Line | [Pakistan Journal of Medical and Health Sciences • 2020]

Author(s):

1. SIMA OROUEI: Department of Genetic Faculty of Advanced Science and Technology, Islamic Azad University,,Tehran Medical Sciences Tehran,Iran

2. MALIHEH ENTEZARI: Department of Genetic Faculty of Advanced Science and Technology, Islamic Azad University,,Tehran Medical Sciences Tehran,Iran

3. MEHRDAD HASHEMI: Department of Genetic Faculty of Advanced Science and Technology, Islamic Azad University,,Tehran Medical Sciences Tehran,Iran

4. KIAVASH HUSHMANDI: Department of food hygiene, Faculty of Veterinary Medicine, Tehran University,Tehran,Iran

5. ABOLFAZL MOVAFAGH: Department of Medical Genetics, cancer Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences,,

Abstract:

Aim: The aim of this project was to investigate the effect of GW9508 and S14161 small molecules on their viability and expression of apoptotic genes in the A549 cell line. Methods:In this experiment, the A549 cell line was first cultured in DMEM medium containing 10% FBS and then treated with different concentrations of both compounds. MTT assay was performed on days 1, 3, and 5 to determine IC50 and to compare the viability of cells treated with different concentrations of GW9508 and S14161. The qRT-PCR assay was employed to investigate the effects of S14161 and GW9508 with IC50 concentration on apoptosis induction, expression of genes including p53, Bax, Bad and Bcl2.Both small molecules induced apoptosis by increasing apoptotic Bax, Bad and P53 gene expression and decreasing Bcl2 gene expression. Results:Comparison between the effect of S14161 and GW9508 individually and simultaneously on cell viability using MTT assay results and gene analysis showed that the combined use of both compounds had a stronger effect on inducing cell death and apoptotic gene expression in a dose- and time-dependent manner, the more time passes the more lethal power it gains. Conclusion:Two small molecules of GW9508 and S14161 activate apoptotic gene expression and inhibit the antiapoptotic gene in two different and parallel pathways, thus being able to act synergistically under concomitant use and have a stronger lethal effect on lung cancer cells.

Page(s): 916-923

DOI: DOI not available

Published: Journal: Pakistan Journal of Medical and Health Sciences, Volume: 14, Issue: 2, Year: 2020

Keywords:

Lung cancer , S14161 , GW9508 , Apoptotic , Cell line

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