The Effects of Magnetic Field and Rhizobial Inoculation on Nitrogen Fixation Process and Growth Parameters in Alfalfa (Medicago sativa L.). | [Pakistan Journal of Botany • 2019]

Author(s):

1. Neda Kazemi Khaledi: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2. SARA SAADATMAND: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3. Ramazan Ali Khavarinejad: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

4. TAHER NEJADSATTARI: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract:

The present study aimed to investigate the effects of magnetic field and rhizobial inoculant on the Alfalfa's growth parameters (leaves, nodules number, shoot and root lengths) and nitrogen fixation process. Inoculated and non-inoculated seed with Sinorhizobium meliloti were subjected to 0.75mT and 1.5mT intensities of magnetic field. Plants were analyzed after 60 days growing. Findings showed 1.5mT magnetic intensity reduced growth parameters, nitrate reductase (NR), nitrite reductase (NIR) and ammonium content, while nitrate and nitrite content increased in comparison with the control group. Application of rhizobial inoculant with 1.5mT intensity increased growth parameters, NR, NIR activity, nitrite, and ammonium content, while it reduced nitrate accumulation in comparison with the single application which was received just 1.5mT intensity. A separate application of 0.75mT intensity increased growth parameters, but had no effect on NR, NIR activity, ammonium, nitrate and nitrite contents, while using rhizobial inoculant individually and combined with 0.75mT intensity increased growth parameters, NR, NIR activity, and ammonium content. Also, it decreased nitrate and nitrite contents as compared to the control. As a result, it could be concluded that rhizobial inoculant application, alone or in combination with a suitable magnetic intensity (0.75mT), could be used as a biofertilizer for vegetable production in sustainable and ecological agricultural systems.

Page(s): 1137-1141

DOI: 10.30848/PJB2019-3(39)

Published: Journal: Pakistan Journal of Botany, Volume: 51, Issue: 3, Year: 2019

Keywords:

nitrate , alfalfa , inoculant , Magnetic field , Nitrite , Ammonium

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