Abstract:
Neonatal pneumonia caused by Burkholderiacepacia(B. cepacia)poses a significant health burden globally includingDistrict Swat, Pakistan. Neonatal sepsis is the main factor in newborn mortality and morbidity causing more than 50% mortality in underdeveloped nations. This study aimed to evaluate the antimicrobial potential of local medicinal plants against multidrug resistant strains of B. cepacia in neonatal pneumonia cases. A total of 160 blood samples were taken neonates suspected pneumonia from the neonatal unit of Saidu Teaching Hospital (STH) in Swat, Khyber-Pakhtunkhwa. The isolates were biochemically identified as B. cepacia using the analytical profile index (API). Further The investigation was carried out to evaluate the antibiotic profile of the isolates. The biochemically characterized isolates were subsequently tested for antibiotic sensitivity using 10 commercial antibiotics according to CLSI standards. The results revealed high frequency of B. cepaciain male children than females. The isolates of B. cepacia were susceptible to Piperacillin/ Tazolactum & Levofloxacin. Doxycycline, Meropenem, & Trimethoprim/sulfamethoxazole, exhibited intermediate activity. A total of ten medicinal plant samples were collected from the region and subjected to extraction using different polar solvents (ethanol, cold distilled Water and hot water). The resulting extracts were then tested for their antimicrobial activity against B. cepacia isolates obtained from neonatal pneumonia cases in the district. The hot boiled water extracts of Euphorbia cotinifolia showed maximum antibacterial activity with 26mm Zone of Inhibition (ZI) followed by Matricaria chamomilla with 25mm ZI. Additionally, Glycyrrhiza glabra, Myrtus communis Withania somnifera, Polygonum bistorta and Ocimum tenuiflorum also showed intermediate activity. While, Fagonia indica, Azadirachta indica, Artemisia vulgaris was recorded ineffective against the isolates. The two most effective medicinal plants with different solvents extracts which showed strong antibacterial potential against the MDR isolates of B. cepacia were then subject to antioxidant assay The radical scavenging assay of the extract revealed that among the tested samples, Matricaria chamomilla in hot water extracts (84.68%) distilled water (75.87 %) and ethanol (65.68%) at 1500ug/mL, followed by Euphorbia cotinifolia in ethanol extract with (46.38%) distilled water (39.41 %) and hot water extracts (38.87%) at the same concentration. Furthermore, the chemical characterization of the active extracts by Florescent Transmission Infra-Red Spectroscopy(FTIR) indicated the presence of various bioactive compounds, including (C=O), (C=C), R-O-R, (O-H), and N-O (stretching/bending). All extracts had the characteristics of C-H stretching, (C=C), and R-O-R, indicating the existence of certain ether, alkane, and alkene compounds. In all extracts, cyclo-hexyl bonding was discovered. These findings highlight the potential of local medicinal plants as a valuable source of antimicrobial agents against B. cepacia in neonatal pneumonia. Further studies are warranted to explore the therapeutic potential of these plant-derived compounds and their application in the development of novel treatment strategies for neonatal pneumonia caused by B. cepacia in the district of Swat.
Page(s):
173-173
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
FTIR
,
medicinal plant
,
Analytical profile index API
,
antibiotic profiling
,
Burkholderia cepacia
,
scavenging assay