Abstract:
Aminated cellulose (ACs) derivatives-based hydrogels are emerging as advanced health care functional materials in biomedicines, but limited data are available regarding its cytotoxicity studies for various biomedical application. In this study we synthesized 6-deoxy-amino-cellulose derivatives named as 6-deoxy-6-hydrazide Cellulose (Cell Hyd) 6-deoxy-6-diethylamide Cellulose (Cell DEA) and 6-deoxy-6-diethyltriamine Cellulose (Cell DETA) from Microcrystalline cellulose (MCC) via tosylation and explored their cytotoxic potential. AC derivatives were synthesized and then characterized by Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), nuclear magnetic resonance spectroscopy (NMR), X-RAY diffractogram (XRD), Scanning Electron microscopy (SEM), Elemental Analysis and Zeta potential measurements. Cytotoxicity was evaluated against normal fibroblasts (NIH3T3), mouse skin melanoma (B16F10), human epithelial adenocarcinoma (MDA-MB-231) and human breast adenocarcinoma (MCF-7) cell lines. IC50 values obtained from cytotoxicity assay and live/dead assay images analysis showed MCC was non cytotoxic while Cell Hyd, Cell DEA and Cell DETA exhibited noncytotoxic activity up to 200 µg/ml to normal fibroblast cells NIH3T3, suggesting its safe use in medical fields. The mouse skin melanoma (B16F10) are the most sensitive cells to the cytotoxic effects of Cell Hyd, Cell DEA, and Cell DETA, followed by human breast adenocarcinoma (MCF-7). MCC and AC derivatives were embedded in methacrylated gelatin (GelMA) for wound healing applications. GelMA and ACs were synthesized and characterized by spectroscopic techniques. Tensile strain of GelMA 61.30 % at break was increased to 64.3% in case of GelMA/CellHYD. In vitro cytocompatibility and cell proliferation using NIH-3T3 cell lines showed cell density trend on scaffold as GelMA/Cell-DETA>GelMA/Cell-Hyd> GelMA. Scratch assay for wound healing revealed that GelMA/CellDETA showed complete wound closure, while GelMA/Cell-Hyd and GelMA exhibited 85.7%, and 66.1% wound healing, respectively in 8 hours. In vivo tests on rats revealed that GelMA/Cell-DETA exhibited 98% wound closure on day 9, whereas GelMA/Cell-Hyd exhibited 97.7% and GelMA 66.1% wound healing on day 14. Based on our study, it is suggested that aminated cellulose derivatives could be promising candidates for tissue engineering applications and in cancer inhibiting studies in future, while GelMA embedded amine MCC derivatives hydrogels can be applied for achieving accelerated wound healing.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
cellulose
,
hydrogels
,
Tissue engineering
,
Biomedicins