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In vitro evaluation of Aloe saponaria as a potential feed additive to reduce protozoal count and methanogenesis in ruminants
Author(s):
1. Muhammad Mahboob Ali Hamid: Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, UAF, Faisalabad, Pakistan
2. Ammara Ammer: Department of Animal Resource, Daegu University, Gyeongbuk 38453, Korea
3. Muhammad Saim Arif: Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, UAF, Faisalabad, Pakistan
4. Ikramah Ashfaq: Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, UAF, Faisalabad, Pakistan
5. Qaswar Hafeez: Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, UAF, Faisalabad, Pakistan
6. Ramish Abrar: Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, UAF, Faisalabad, Pakistan
Abstract:
This study was conducted to evaluate dietary supplementation of Aloe saponaria (AS) on rumen fermentation and rumen microbial diversity in in vitro. Two different forms of AS (heat dried [HAS] and freeze dried [FAS]) were prepared and five treatments were used for in vitro fermentation over a 48-h incubation period: no addition of AS (CON), 1% and 2% addition of HAS (HAS1, HAS2, respectively), and the same level of FAS addition (FAS1, FAS2 respectively). Significantly higher (P < 0.05) gas production was observed in AS treatments than CON during the incubation. The addition, levels, and drying methods of the AS did not affect (P > 0.05) the production of methane and gas fitted parameters. The in vitro nutrient degradability, pH and ammonia-N concentrations of AS treatments did not differ (P > 0.05) from CON. AS addition and levels significantly increased (P < 0.05) the total VFA, acetate proportion, general bacteria, fungi, and methanogenic archaea but the freeze dried AS decreased ciliate protozoa (P < 0.05) abundance. In conclusion, freeze dried AS can reduce rumen ciliate protozoa and safely manipulate the ruminant fermentation characteristics. 
Page(s): 67-67
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Drying methods , methane production , In vitro fermentation , rumen microbes , Aloe saponaria
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