Administration of Bee Pollen,Nigella sativa Oil,and their Combination as a Strateg y for Improving Growth Performance, Immunity, and Health Status of Newborn Friesian Calves During the Suckling Period | [Journal of animal health and production • 2023]

Author(s):

1. H.A. El-Nagar: Animal Production Research Institute, Agricultural Research Center, Giza, Egypt

2. A.M. El-Hais: Animal Production Department, Faculty of Agriculture, Tanta University, Egypt

3. M.S. Mandouh: Animal Production Department, Faculty of Agriculture, Tanta University, Egypt

4. W.M. Wafa: Animal Production Research Institute, Agricultural Research Center, Giza, Egypt

5. A.H. ABD El-Aziz: Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Damanhour University, Egypt

6. K.A. Attia: Department of Evaluation of Natural Resources, Environmental Studies and Research Institute, University of El Sadat City, El-Sadat City, 32897, Egypt.

Abstract:

| This study aimed to evaluate the efect of dietary supplementation of bee pollen (BP), black seed oil (BS, Nigella sativa), or their combination on growth performance, immunity, and health status of females of newborn Friesian calves. A total of 20 newborn calves (37.51±0.92 kg LBW ) were divided into 4 groups. During the suckling period, animals in the 1st group were fed a starter without any addition (G1, control). Animals in G2, G3, and G4 were fed the same starter supplemented with BP (10g/kg), BS oil (10g/kg), or their combination (5g Bp+5g BS oil), respectively. Results showed that all immunoglobulin types (IgG, IgM, and IgA), total protein, albumin, globulin, glucose, total lipids, total cholesterol, and total antioxidant capacity in blood serum, and RBCs, WBCs, Hb, and PCV increased (P<0.05) in treated than in the control calves, being the highest with the combination treatment. Urea-N and creatinine concentrations, and AST and ALT activities decreased (P<0.05) in treated compared with the control calves, with the lowest values for the combination treatment. The treated calves were heavier with higher weight gains than the control one, being the highest with the combination treatment. In conclusion, addition of a combination of 5g bee pollen and 5g black seeds oil/kg in the starter during the sucking period improves live body weight, weight gain, immunity, kidney and liver functions, and antioxidant status of female newborn Friesian calves. This administration is considered a good strategy for raising dairy calves in dairy farms.

Page(s): 306-316

DOI: 10.17582/journal.jahp/2023/11.3.306.316

Published: Journal: Journal of animal health and production, Volume: 11, Issue: 3, Year: 2023

Keywords:

Bee pollen , hematology , Immunoglobulins , | Friesian calves , Black seeds , Blood constituents

References:

[1] El-Hafeez A.M.; Ali Abd,El-Hamd M.A.; Wahba M.A.E.; Abu,Kamla A.A.,M.E. A.A. .2014 .Productive performance, immune status and metabolic activity of suckling bovine calves treated with Nigella sativa oil. Egypt. J. Agric. Res., 157682(4) : 1561-1574.

[2] Abdel-Magid S ,El-Kady R.I.; Gad S.M.,Awadalla I.M. .2007 .Using cheap and local non-conventional protein meal (Nigella sativa) as least cost rations formula on performance of crossbreed calves. Int. J. Agric. Biol, 9 : 1560-8530.

[3] Abdel-Raouf E.M.; Mohsen M.K.; Gaafar H.M. ,Mesbah R.A. .2018 .Blood Biochemical, Haematological, Immunity Response and Diarrhea Incidence of Early Weaning Friesian Calves Supplemented with Bee Pollen and Black Seeds Int. J. Res. Stud. Agricult. Sci., 4(9) : 0409004-6224.

[4] Ibrahim Adetuyi F.,T. Adetuyi F. .2014 .Efect of fermentation time on the phenolic, flavonoid and vitamin C contents and antioxidant activities of okra (Abelmoschus esculentus) seeds. , 7241(15) : 30128-4.

[5] Bassuony Ahmed A.S.,Awad S. Set I. Neamat,Mohamed A.M.,S.A. A.M. .2009 .Adding Natural Juice of Vegetables and Fruitage to Ruminant Diets (B) Nutrients Utilization, Microbial Safety and Immunity, Efect of Diets Supplemented with Lemon, Onion and Garlic Juice Fed to Growing Bufalo Calves. World J. Agricult. Sci., 5(4) : 456-465.

[6] Deger Aliyzicioglu Y.,Ovali O.,Hosver E.,Tekelloglu I.,Karaman Y.,S.C. Y. .2005 .Efect of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. In Int. Immunopharmacol., 04(11) : 1652-1657.

[7] Anjum M.I.,Azim A.,Jabbar M.A.; Anwar M.,Mirza I.H. .2012 .Age and Weight at Puberty in Nili-Ravi Bufalo Heifers Reared on Three Dietary Energy Restriction Periods followed by Compensatory Growth. , 32 : 367-371.

[8] Atta M.B. .2003 .Some characteristics of nigella (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chem, 8146(03) : 00038-4.

[9] Al-Hamid Attia Y.A.; Abd,Ibrahim A.E.,Al-Harthi M.A.; Bovera M.S.,Elnaggar F.,A.Sh. F. .2014 .Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen, and mannan oligosaccharides continuously or intermittently. Livest. Sci., 03 : 87-95.

[10] Boyd J.W. .1984 .The interpretation of serum biochemistry test results in domestic animals. Vet, 13(2) : 165x-14.

[11] Butt M.S.,Sultan M.T. .2010 .Nigella sativa: reduces the risk of various maladies. Crit. Rev. Food Sci. Nutr, 50(7) : 654-65.

[12] Cai Y.; Sun M.,Corke H. .2003 .Antioxidant activity of betalains from plants of the amaranthaceae. J. Agric. Food Chem, 9(8) : 2288-2294.

[13] Dubois Cara L.,Borel P.; Armand M.; Senft C.,Portugal H.; Pauli A.M.; Bernard M.,Lairon P.M.,D. P.M. .1992 .Efects of oat bran, rice bran, wheat fiber, and wheat germ on postprandial lipemia in healthy adults. Am. J. Clin. Nutr, 55(1) : 81-88.

[14] Stanford Chaves A.V.,Dugan M.E.R.; Gibson L.L.; McAllister T.A.; Van Herk K.,Benchaar F.,C. F. .2007 .Efects of cinnamaldehyde, garlic and juniper berry essential oils on rumen fermentation, blood metabolites, growth performance, and carcass characteristics of growing lambs. Livest. Sci., 12(2-3) : 215-224.

[15] Cherif M.; Ben Salem H.,Abidi S. .2017 .Efect of the addition of Nigella sativa seeds to low or high concentrate diets on intake, digestion, blood metabolites, growth and carcass traits of Barbarine lamb. Small Rumin. Res., 11 : 1-8.

[16] Cowan M.M. .1999 .Plant products as antimicrobial agents. Clin. Microbiol, 12(4) : 564-582.

[17] Fawzy Darwish A.A.,Osman M.,El Ebissy W.A.,E.A. W.A. .2021 .Clinicopathological and bacteriological studies on lamb bacterial enteritis and monitoring the oregano oil and vitamins A, D3, E efect on its treatment. J. Adv. Vet. Anim. Res., 8(2) : 291-299.

[18] De Oliveira M.C.; da Silva D.M.; Loch F.C.; Martins P.C.; Dias D.M.,Simon G.A. .2013 .Efect of bee pollen on the immunity and tibia characteristics in broilers. Rev. Brasil. de Ciência Avícola, 15 : S1516-327.

[19] Desai S.D. ,Sh. H. Saheb; K.K. Das S.,Haseena S. .2015 .Phytochemical analysis of Nigella sativa and it`s antidiabetic efect. J. Pharm. Sci. Res, 7(8) : 527-532.

[20] Dorucu M.S.,Colak O.,Ispir U.,Altinterim B.,Celayir Y. .2009 .The Efect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout. , 2667(1) : 27-33.

[21] Doumas B.T.,Wabson W.A.,Biggs H.G. .1971 .Albumin standards and measurements of serum with bromocresol green. Clin. Chem. Acta, 1997 Feb, 8981(96) : 06447-9.

[22] El-Gaafrawy A,Ahmed M.Nagwa A,El-Banna M.K.,Ibrahim I.L. .2000 .Efect of Selenium and vitamin E supplementation on immue response and performance of Baladi calves. Proc. Conf. Anim. Prod. In the 21th Century, Sakha, : 267-276.

[23] El-Hanoun A.M.; H. Hedia; M.S. El-Sbeiy K.I. Kamel .2010 .Efect of bee pollen supplementation on some productive, reproductive and biochemical traits of growing male rabbits during winter and summer seasons. 5th Int. Conf. on Rabbit Prod. in Hot Seasons, 01054 : 0396-433.

[24] El-Neney ,A. M. ,El-Kholy K.H. .2013 .Efect of natural additive (bee pollen) on immunity and productive and reproductive performances in rabbits. 1- Growth performance, digestive and immune responses in growing rabbits. Egypt. Poult. Sci., 32572 : 579-606.

[25] El-Sisy G.A.; Khalifa W.H.,El-Nattat W.S.; Abusinaa G.E.,Maghraby N.A. .2018 .Efect of dietary supplementation of wheat germ on some reproductive performances and oxidative status of rabbit bucks under heat stress. Biosci. Res, 15(4) : 4267-4273.

[26] Farag Soha A.,El-Rayes T.K. .2016 .Efect of bee pollen supplementation on performance, carcass traits and blood parameters of broiler chickens. Asian J. Anim. Vet, 168 : 168-177.

[27] El Habeeb A.A.M.,Tarabany A.A. Habeeb A.A.M. .2012 .Efect of Nigella sativa or curcumin on daily body weight gain, feed intake and some physiological functions in growing Zaraibi goats during hot summer season. In Proceedings of the Third International on Radiation Sciences and Applications, : 920-16.

[28] Halliwell B. .1990 .How to characterize a biological antioxidant. Free Radic. Res. Commun. 9, : 1-32.

[29] Hannan J.M.A.; Ansari P.; Haque A.,Sanju A.,Huzaifa A,Rahman A,Ghosh A.,Azam S. .2019 .Nigella sativa stimulates insulin secretion from isolated rat islets and inhibits the digestion and absorption of (CH2O) in the gut. Biosci. Rep. Aug, 23(8) : .

[30] Henry J.B. .2001 .Clinical Diagnosis and Management by Laboratory Methods. Twentieth Edition, Page, 485 : .

[31] Henry R. ,Publisher Row .1974 .Kinetic determination of serum creatinine. Clinical Chemistry, Principles and Techniques, : 525.

[32] Henry R.J. .1964 .. , : 181.

[33] Xuan H.; Zhu W.; Chen Hu F.,Ying M.,H. M. .2003 .Efects of pollen and propolis on diabetes mellitus SD rats. Apicult. China, 54 : 9-11.

[34] IBM SPSS .2017 .-cite-ibm-spss-statistics-orearlierversions-spss). Version, 25 : .

[35] Izuta H.; Shimazawa M.; Tsuruma K.,Araki Y.,Mishima S.,Hara H. .2009 .Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells. BMC Complement Altern Med, 17(9) : 1472.

[36] Kaneko J.J.; Harvey J.W.,Bruss M.L. .1964 .Clinical biochemistry of the domestic animals. , 03060210073034 : .

[37] Khalifa S.; Elashal M.,Yosri N.,Du M.,Musharraf S.,Sarker S.; Guo Z.; Cao W.,Zou X.; Abd,El-Wahed X.; Abd,Xiao J.; Omar H.A.; Hegazy M.F.,El-Seedi H.R. M.F.,Potential Therapeutic .1876 .Bee Pollen: Current Status. , 13 : .

[38] Khattab H.M.; El‐Basiony A.Z.; Hamdy S.M.,Marwan A.A. .2011 .(www.ijas. Iranian J. Appl. Anim. Sci. 1, (4) : 227-234.

[39] Savory Killingsworth L.M.,J Killingsworth L.M. .1972 .Manual Nephelometric Methods for Immunochemical determination of immunoglobulins IgG, IgA. Clin. Chem, 18(4) : 335-339.

[40] Koracevic D.; Koracevic G.; Djordjevic V.,Andrejevic S.,Cosic V. .2001 .Method for the measurement of antioxidant activity in human fluids. J. Clin. Pathol, 54(5) : 356-361.

[41] Lee I.K.,Hwang B.S.,Kim J.Y.; Woo E.E.,Lee Y.J.; Choi H.J. E.E.,Yun B.S. .2016 .Characterization of Neuraminidase Inhibitors in Korean Papaver rhoeas Bee Pollen Contributing to Anti-Influenza Activities In Vitro. Planta Med, 82(6) : 524-9.

[42] Lee K.-W.; Everts H.,Beynen A.C. .2004 .. Essential Oils in Broiler Nutrition. Int. J. Poult. Sci., 738 : 738-752.

[43] Mahmoud H.S.; Almallah A.A.; Gad,El-Hak H.N.,Aldayel T.S.,Abdelrazek H.M. T.S.,Khaled H.E. .2021 .The efect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats. Sci. Rep, 11(1) : 7542.

[44] Ndlovu T.,Chimonyo M.; Okoh A.I.,Dzama K.,Raats J.G.,Biotechnol Afr. J. .2007 .Assessing the nutritional status of beef cattle: current practices and future prospects-Review.. , 6(24) : 000-2436.

[45] Obeidat B.S. .2020 .The Inclusion of Black Cumin Meal Improves Growth Performance of Growing Awassi Lambs. , 7(2) : 40.

[46] Crouch Patton G.,S. Patton G. .1977 .Colorimetric Method for the Determination of Serum Urea. , 49 : 464-469.

[47] Platel K. Srinivasan K. .2000 .:AIDFOOD42>3.0. Food/Nahrung, 44(20000101) : 42-3803.

[48] Quezada-Mendoza V.C.,Heinrichs A.J.,Jones C.M. .2011 .hTe efects of a prebiotic supplement (Prebio Support) on fecal and salivary IgA in neonatal dairy calves. Livest. Sci, 07(1) : 222-228.

[49] Raboisson D.; Delor F.; Cahuzac E.,Gendre C.,Sans P.,Allaire G. .2013 .Perinatal, neonatal, and rearing period mortality of dairy calves and replacement heifers in France. , 6010(5) : 2913-2924.

[50] Reitman S. Frankel S. .1957 .A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol, 28(1) : 56-63.

[51] Retnani Y.; Wiryawan K.G.; Khotijah L.; Barkah N.N.,Gustian R.A.; Dermawan I.R. .2019 .Growth performance, blood metabolites and nitrogen utilization of lambs fed with Nigella sativa meal. Pak. J. Nutr, 247 : 247-253.

[52] Ribeiro M.D.; Pereira J.C.; Queiroz A.C.de.; Cecon P.R.; Detmann E.,Azevêdo J.A.G. .2009 .Performance of dairy calves fed milk, milk replacer or post-weaning concentrate with acidifiers. Rev. Brasil, 38(5) : 956-963.

[53] Richmond W. .1973 .its application to the enzymatic assay of total cholesterol in serum. Clin. Chem, 19(12) : 1350-1356.

[54] El-Awady Salem A.,Tag EL-Dein H.,Eisa M.,D. M. .2019 .(22-Suppl). , 56 : .

[55] Salim ,Hanim K.,Ahmed H.A.,Alexandria J.,Vet J. .2019 .Growth performance, blood biochemical constituents, antioxidant status, and meat fatty acids composition of lambs fed diets supplemented with plant essential oils. , 63(2) : 156-165.

[56] Sarker M.S.K.; Ko S.Y.; Lee S.M.; Kim G.M.; Choi J.K.,Yang C.J. .2010 .Efect of Diferent Feed Additives on Growth Performance and Blood Profiles of Korean Hanwoo Calves. Asian-Australasian Journal of Animal Sciences. Asian Australasian Assoc. Anim. Prod. Societ., 90280 : 52-60.

[57] Abd-El-Sater Shalaby A.,Abdel-Hamid G.; Mahmoud K.,A.M. K.,El-Deen Nour,A.S.S. Nour,Med Nour .2016 .Changes in lipid profile, AST, ALT, urea, and creatinine by Nigella sativa seeds powder in adult male albino diabetic rats. , 45(1) : 13-22.

[58] Van Amburgh Soberon F.,M.E. Soberon F. .2013 .Lactation Biology Symposium: The efect of nutrient intake from milk or milk replacer of preweaned dairy calves on lactation milk yield as adults: a meta-analysis of current data. J. Anim. Sci., 5834(2) : 706-712.

[59] Soberon F.; Rafrenato E.,Everet R.W.,Van Amburgh M.E. .2012 .Preweaning milk replacer intake and efects on longterm productivity of dairy calves. J. Dairy Sci., 95 : 4391-793.

[60] Söderholm J.D ,Perdue M.H. .2001 .Stress and gastrointestinal tract. II. Stress and intestinal barrier function. Am. J. Physiol. Gastrointest Liver Physiol, 280(1) : G7-G13.

[61] Sreedhar S.; Ranganadham M.,Mohan E.M. .2010 .Calf mortality in indigenous bufaloes. Indian Vet. , 87 : 197-198.

[62] Srinivasan K.,Sambaiah K. .1991 .The efect of spices on cholesterol 7 alpha-hydroxylase activity and on serum and hepatic cholesterol levels in the rat. Int. J. Vitam. Nutr. Res., 61(4) : 364-369.

[63] Taha E.A. .2017 .A study of some wool traits of Barki sheep fed on Nigella sativa cake under desert conditions. Alexandria J. Agricult. Res., 62(4) : 341-348.

[64] Tassoul M.D. ,Shaver R.D. .2009 .Efect of a mixture of supplemental dietary plant essential oils on performance of periparturient and early lactation dairy cows. J. Dairy Sci., 1760(4) : 1734-1740.

[65] Trinder P. .1969 .Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J. Clin. Pathol, 22(2) : 158-161.

[66] Deng Tu Y.; Zhang G.F.,Diao K.D.; Zhang N.F.,Q.Y. K.D.; Zhang N.F. .2015 .Efects of supplementary bee pollen and its polysaccharides on nutrient digestibility and serum biochemical parameters in Holstein calves. Anim. Prod. Sci., 55 : 1318-1323.

[67] El-Tawab Wafa W.M.,Bedawy A.A.A.; Elhofy F.I.,Y.M. A.A.A.; Elhofy F.I. .2021 .Immune response, health status, blood components and growth performance of suckling calves treated with thymus vulgaris extract. J. Anim. Health Prod., 9(3) : 342-351.

[68] Wang J.; Li S.,Wang Q.; Xin B.,Wang H. .2006 .Trophic efect of bee pollen on small intestine in broiler chickens. J. Med, 215(2) : 276-280.

[69] Xu X.,Dong J.,Zhang H .2008 .Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon oxide. Innovat. Food Sci. Emerg, 08(1) : 42-46.

[70] Yang W.Z.; Ametaj B.N.,Benchaar C.,Beauchemin K.A. .2010 .Dose response to cinnamaldehyde supplementation in growing beef heifers: ruminal and intestinal digestion. J. Anim. Sci., 1652(2) : 680-688.

[71] Shams Zaki Q.,Jarjeis E.A. Zaki Q. .2015 .Evaluation of using some Medical Herbs Seeds as Feed Additive on some Hematological and Biochemical Parameters for Male Awassi Lambs Under Local Environmental Condition of Nineveh Province, IRAQ. Basic Appl. Sci., 9(20) : 527-537.

[72] Kirsch Zöllner N.,K. Zöllner N. .1962 .Colorimetric Method for Determination of Total Lipids. J. Experimen. Med, 135 : 545-550.

Citations

Downloads

Views