Abstract:
Sinusitis is an inflammation of the membranes lining the paranasal sinus, one of the most frequently diagnosed diseases in the United States. Bacteria in it are highly resistant to antibiotics and scientists are looking for medicinal plants as potent biofilm agents. The biological activities such as biofilm, antibacterial, mutagenicity and toxicity of extracts were checked by microtiter plate method, well diffusion, Ames assay and hemolytic analysis respectively. The current study was aimed to develop a natural product against sinusitis from five plant extracts. Minimum hemolytic activity was shown by water (3.37 ± 0.102) and ethanol (8.27 ± 0.129) extracts while the highest anti-inflammatory activity was shown by n-Hexane (49.35 ± 0.40) extract. Increased thrombolytic activity was observed in the case of ethanol (80.31 ± 0.13) and the lowest by n-Hexane (56.82 ± 0.06) extract. Antibacterial activity of ethanolic extract was remarkable followed by water extract against all tested strains (Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Staphylococcus aureus, Enterococcus faecalis) and consortium from human volunteer. The value of minimum inhibitory concentration for ethanolic extract ranged from 0.625 to 2.5 mg/mL for tested bacterial strains and consortium. Biofilm inhibition studies have shown that maximum biofilm inhibition was observed in the case of n-Hexane extracts and ethanol extracts. Ames assay was also performed, and results revealed that these extracts are non-mutagenic as well as non-toxic. Statistical analysis was also performed using one way ANOVA. Results of this study have shown that (polyherbal drug) product developed in different extracts have lowest toxicological, remarkable antibacterial and biofilm inhibition activity. This product is safe to use as its toxicological and biological profile is very good.
Page(s):
104-104
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords:
biofilm
,
antibacterial
,
Sinusitis
,
Hemolytic
,
Polyherbal product