Abstract:
Alzheimer`s disease (AD) is a prevalent neurodegenerative condition that is characterized by a reduction in cognitive ability as well as disrupted neural connections. Damage caused by oxidative stress as well as neuroinflammation are two of the most important contributors to the onset of Alzheimer`s disease (AD). Natural compounds possessing anti-inflammatory and antioxidant characteristics have emerged as potential therapy possibilities. The objective of the present study was to investigate the potential synergistic effects of Apigenin and Safranal on oxidative damage and neurological dysfunction in rats affect with Alzheimer`s disease (AD). In this study, 36 albino Wistar rats were divided into six groups. The healthy control group received intraperitoneal injection of normal saline. The disease control group was intraperitoneally injected with scopolamine (2.5 mg/kg). Donepezil group, Apigenin group, Safranal group and API+SAF group also received intraperitoneal injection of scopolamine (2.5 mg/kg), followed by oral administration of donepezil (1.5 mg/kg), Apigenin (50mg/kg) and Safranal (0.2ml/kg) and API+SAF (Safranal (0.2 ml/kg) and Apigenin (50mg/kg)) respectively. The study lasted four weeks, after which cognitive function and behavioral changes were assessed. After analysis, rats were euthanized and specific brain regions collected for neurochemical assessment. Various biomarkers related to oxidative stress and brain function were measured, such as MDA, SOD, catalase, GPx, GSH, total protein content and acetylcholine levels. The results showed that the combination of Apigenin and Safranal exerted a significant neuroprotective effect compared with the disease control group and the treatment group alone i.e., Apigenin and Safranal. Furthermore, the synergistic group showed a reduction in oxidative stress, as indicated by decreased MDA levels and increased SOD, catalase, GPx and GSH activities. Behavioral tests, including the open field test (OFT), light & dark box (LADB), elevated plus maze test (EPMT), Kondziela’s inverted screen test (IST), forced swim test (FST), pole test (PT) were conducted to assess cognitive function ,depression and anxiety. Also showed a significant increase in acetylcholine levels.
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Published:
Journal: Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 , Volume: 0, Issue: 0, Year: 2023