Variance Analysis of Intestinal Microbial Diversity of the Noble Scallop (Chlamys nobilis) under Enrofloxacin Exposure | [Pakistan Journal of Zoology • 2021]

Author(s):

1. Jiawei Hong: Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China

2. Mingqiang Chen: Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China

3. Zhenghua Deng: Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China

4. Youning Li: Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China

5. Yu Wang: Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China

Abstract:

To investigate the possible impact of enrofloxacin (ENR) on noble scallop (Chlamys nobilis), we quantitatively evaluated the microbial shifts in the intestine of noble scallop in response to enrofloxacin treatments at different dosages (0, 5, and 10mg/L ENR) using 16S rDNA gene sequencing. A total of 11 phyla comprising 76 genera were detected. At the phylum level, the relative abundance of Proteobacteria increased (from 34.96% to 77.31%) with the increasing of enrofloxacin exposure dosage. The dominant position of Tenericutes was replaced by Proteobacteria, and in parallel the proportion of Tenericutes slumped to 3.85%. At the genus level, the relative abundance of Mycoplasma, dropped down from 58.38% to 3.85%, and Vibrio increased (from 15.23% to 40.8%) to become the dominant genus. The hierarchical clustering heat map analysis and principal component analysis (PCA) showed that the microbial community of the high dosage group (10 mg/L) was clearly different from the other two groups. Overall, enrofloxacin at high dosage of 10 mg/L significantly altered the community diversity of noble scallop. This study characterized the variation regularity of the intestinal microbial of the noble scallop in response to enrofloxacin treatment. These results, provide a comprehensive acquaintance with intestinal microecosystem of the noble scallop and contributes to a reasonable use of enrofloxacin treatment on noble scallop.

Page(s): 1221-1231

DOI: 10.17582/journal.pjz/20190425190440

Published: Journal: Pakistan Journal of Zoology, Volume: 53, Issue: 4, Year: 2021

Keywords:

Chlamys nobilis , microbial diversity , enrofloxacin , 16S rDNA

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