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Total protein polymorphism of some isolates of fusarium oxysporum f. Sp. Albedinis using sds-page
Author(s):
1. Sidaoui Abouamama: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria; Faculty of Science and Technology, Department of Biology, Amine Elokkal El HadjMoussa Egakhamouk University of Tamanrasset, 11000 Tamanrasset, Algeria.
2. Karkachi Noureddine: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria.
3. Bertella Anis: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria; Department of Molecular and Cellular Biology, Faculty of Nature and Life Sciences, Abbes Laghrour University of Khenchela, Algeria.
4. Haouhach Sadika: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria Department of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, 31000 Oran, Algeria;
5. Gharbi Samia: Department of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, 31000 Oran, Algeria
6. ElGoumi Younes: Faculty of Science and Technology of Settat Hassan 1st University –Morocco
7. Terbeche Rym: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria; Department of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, 31000 Oran, Algeria
8. Kihal Mebrouk: Laboratory of Applied Microbiology, Department of Biology. University of Oran 1 (Ahmed Ben Bella), B.P. 1524, El M Naouer- 31000.Oran, Algeria
Abstract:
Fusarium wilt caused by Fusarium oxysporum f.sp. albedinis (Foa), is one of the main diseases affecting date palm (Phoenix dactylifera L.), and poses a constant threat to palm oases, especially in North African countries. In this study, we performed electrophoresis of total proteins extracted from the mycelia of 20 Fusarium oxysporum f.sp. albedinis and 03 isolates of Fusarium sp. Protein was extracted and separated by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis followed by staining with silver nitrate. We noted variations in protein profile between the 23 Fusarium strains isolated from three regions Ghardaïa, Bechar and Adrar. These results demonstrate the presence of important phenotypic polymorphism according to the ascending hierarchical classification of total proteins after the analysis of the phonograms constructed by the JMP software (JMP SAS Pro (JMP®, Version <12>. SAS Institute Inc., 2015).
Page(s): 7-15
Published: Journal: Pakistan Journal of Phytopathology, Volume: 33, Issue: 1, Year: 2021
Keywords:
Fusarium oxysporum f , sp , Electrophoresis , phenotypic polymorphism , albedinis , Total Protein
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