Thermal stable and proteinase-K resistant insecticidal toxins produced by Photorhabdus luminescens | [Abasyn Journal of Life Sciences • 2021]

Author(s):

1. Ihsan Ullah: Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

2. Khalid M.S. Al-ghamdi: Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

Abstract:

Photorhabdus is lives in a mutualistic association with nematodes from the family Heterorhabditis. Bacteria of the Photorhabdus can survive independently and cause toxicity in a larger variety of insects. In the present study, insecticidal activity of non-portentous heat-stable metabolites of Photorhabdus luminescens was evaluated against Galleria mellonella. For this purpose, the culture extract of P. luminescens was injected into the G. mellonella larvae, which killed almost 90% of larvae within 48 h. The extract showed 100% insecticidal activity after heat treatment of 70°C for 30 min and even 60% and 40% activity lasted at 80°C and 90°C respectively. The extract also showed a high degree of thermal stability and was 100% actives after 60 min at 70°C. In addition, insecticidal activity was preserved up to 100% after all proteinase-K treatments (0 µg/mL to 50 µg/mL). The results revealed that the extracts were nonportentous and showed high thermal resistance and stability.

Page(s): 161-167

DOI: 10.34091/AJLS.4.1.19

Published: Journal: Abasyn Journal of Life Sciences, Volume: 4, Issue: 1, Year: 2021

Keywords:

Toxins , insecticidal activity , heat stable nonproteinaceous , Photorhabdus

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