UV-B radiation inhibits the photosynthetic electron transport chain in Chlamydomonas reinhardtii. | [Pakistan Journal of Botany • 2016]

Author(s):

1. Wenkai Cai: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA; University of Chinese Academy of Sciences, Beijing, CHINA

2. Xiang Gao: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA; University of Chinese Academy of Sciences, Beijing, CHINA

3. Jinlu Hu: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA; University of Chinese Academy of Sciences, Beijing, CHINA

4. Lanzhou Chen: School of Resource and Environmental Science, Wuhan University, Wuhan, CHINA

5. Xiaoyan Li: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA

6. Yongding Liu: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA

7. Gaohong Wang: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CHINA

Abstract:

UV radiation of sunlight is one of harmful factors for earth organisms, especially for photoautotrophs because they require light for energy and biomass production. A number of works have already been done regarding the effects of UV-B radiation at biochemical and molecular level, which showed that UV-B radiation could inhibit photosynthesis activity and reduce photosynthetic electron transport. However quite limited information can accurately make out inhibition site of UV-B radiation on photosynthetic electron transport. In this study, this issue was investigated through measuring oxygen evolution activity, chlorophyll a fluorescence and gene expression in a model unicellular green alga Chlamydomonas reinhardtii. Our results indicated that UV-B radiation could evidently decrease photosynthesis activity and inhibit electron transport by blocking electron transfer process from the first plastoquinone electron acceptors QA to second plastoquinone electron acceptors QB, but not impair electron transfer from the water oxidizing complex to QA. The psbA gene expression was also altered by UV-B radiation, where up-regulation occurred at 2, 4 and 6h after exposure and down-regulation happened at 12 and 24 H after exposure. These results suggested that UV-B could affects D1 protein normal turnover, so there was not enough D1 for binding with QB, which may affect photosynthetic electron transport and photosynthesis activity.

Page(s): 2587-2593

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 48, Issue: 6, Year: 2016

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