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Therapeutic effect of Earthworm Powder on the Pathogenesis of Entamoeba Histolytica in Vivo.
Author(s):
1. Nebrass F Chachain: College of Science, University of Al-Mustansirya, Iraq
2. Hussein A Jamil: College of Science, University of Al-Mustansirya, Iraq
Abstract:
This study was carried out in vivo to detect the effect of earthworm powder on the Entamoeba histolytica in white mice. The mice infected with the parasite were inoculated by earthworm powder with a concentration of 200 mg/ml for 11 days, during this period, the faeces were examined daily to observe the change in parasite numbers after inoculating with powder. Results showed that the earthworm powder was effective in reducing parasite numbers in mice gradually and when compared the therapeutic efficacy of the earthworm powder with the metronidazole where it was close (61.5 & 63.1) respectively. Histopathological change was study found that the parasite and metronidazole cause necrosis, infiltration of lymphocyte and mucosal damage and while powder of earthworm effectively in repair the damaged intestinal walls. In addition, there was an increased concentration of sIgA in the intestinal tissue, which was concentrated after 3,7,9 days in the treatment earthworm powder group (4.084 ± 1.29, 2.588 ± 0.82, 2.563 ± 0.81) ng/ml respectively. while in the metronidazole after 3,7,9days 4.057±1.28, 2.553±0.81, 2.528±0.71ng/ml respectively compared with control negative group. Also in the earthworm powder group (none infected) it noted after 3,7,9 days (2.064 ± 0.65, 2.17 ± 0.681.901 ± 0.60) ng/ml respectively, and in positive control group the concentration of IgA was 3.221±1.01, 3.370±1.06, 3.580±1.13ng/ml respectively after 3,7,9 days compared with control negative group.
Page(s): 643-651
DOI: DOI not available
Published: Journal: Pakistan Journal of Biotechnology, Volume: 14, Issue: 4, Year: 2017
Keywords:
Keywords are not available for this article.
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