The Cytotoxic Effects of Purple Nutsedge (Cyperus Rotundus L.) Tuber Essential Oil on the Hela Cervical Cancer Cell Line. | [Pakistan Journal of Biotechnology • 2018]

Author(s):

1. Susianti Susianti: Department of Histology, Faculty of Medicine, Lampung University, Bandar lampung, Indonesia

2. Yanwirasti Yanwirasti: Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia

3. Eryati Darwin: Department of Histology, Faculty of Medicine, Andalas University, Padang, Indonesia

4. Jamsari Jamsari: Faculty of Agriculture, Andalas University, Padang, Indonesia

Abstract:

Many problems in cancer therapy have prompted researchers to explore natural materials to discover new anticancer substances with higher efficacy and more minimal side effects. Purple nutsedge (Cyperus rotundus L.) is one of the potential medicinal plants being studied as an anticancer substance. A variety of studies have been conducted on the use of the purple nutsedge tuber as an anticancer substance, but not about the effects of its essential oils on cervical cancer. The method used in this research was a cytotoxic test on HeLa cervical cancer cells using an MTT assay. The cells were incubated with purple nutsedge essential oil in a 96-well plate with eight series of doses (3.9-500 µg/ml), and each dose was performed three times. After the absorbance of the cells was measured using an ELISA reader, the percentage of cell viability was calculated for each dose, followed by the calculation of the inhibitory concentration 50% rate (IC50) using probit regression analysis. This research conclude that purple nutsedge essential oil IC50 on HeLa cells is 35.062 + 11.258µg/ml. This indicates that there is a cytotoxic effect of purple nutsedge essential oil on the HeLa cervical cancer cell line.

Page(s): 77-81

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 15, Issue: 1, Year: 2018

Keywords:

Keywords are not available for this article.

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