Transcriptome analysis of pigment related genes in colored cotton. | [International Journal of Agriculture and Biology • 2015]

Author(s):

1. Waqas Malik: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

2. Asif Ali Khan: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

3. Hafiza Masooma Naseer Cheema: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

4. Usman Aslam: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

5. Muhammad Zaffer Iqbal: Agricultural Biotechnology Research Institute, AARI, Faisalabad, Pakistan

6. Abdul-Qayyum: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

7. Azra Yasmeen: Department of Agronomy, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

8. Noreen Bibi: National Institute of Agriculture and Biology, Faisalabad, Pakistan

Abstract:

The biochemical basis of pigmentation has been well studied in naturally colored cotton but little is known about the molecular basis of color development in cotton fibers. The differences in pigmentation between brown, green and white cotton fiber can be mined by the molecular study of structural genes of flavonoid biosynthesis pathway. The transcriptome analysis of five flavonoid biosynthesis related structural genes, encoding chalcone isomerase (GhCHI), flavanone 3-hydroxylase (GhF3H), dihydroflavonol 4-reductase (GhDFR), anthocyanidin synthase (GhANS), and anthocyanidin reductase (GhANR) was performed at two fiber developmental stages i.e. 10 DPA and 20 DPA in brown, green and white cotton. The transcript level of all structural genes was higher in brown cotton fiber as compared to green and white at both 10 DPA (days post anthesis) and 20 DPA. The decline in the transcript level of all structural genes except GhANS was observed at 20 DPA as compared to 10 DPA. The transcript accumulation of GhANS was negligible in green and white cotton fibre at both stages. Correlating these findings with the flavonoid biosynthetic pathway revealed that GhANR and its substrate GhANS are crucial for the synthesis of proanthocyanadines, which is a brown pigment and transcripts of structural genes are directly proportional to the brown pigment. In conclusion, GhANS gene is strongly associated to the brown color development and modulation in the transcript level of this gene could improve the pigmentation of brown cotton fibers.

Page(s): 205-210

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 17, Issue: 1, Year: 2015

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views