Author(s):
1. Khadija Amjad:
Department of Plant Biotechnology, ASAB, NUST, Islamabad, Pakistan
2. Muhammad Faraz Bhatti:
Department of Plant Biotechnology, ASAB, NUST, Islamabad, Pakistan
Abstract:
Microplastics are irregularly shaped synthetic particles having a size of 1 to 5µm with an annual production of more than 348 million tons. Considerable pollution of the environment has been done due to an increase in plastic production in the past decades. These pose a significant threat to the environment. In this study, various ascomycetes were explored for lipase that is capable of microplastic degradation. After a comprehensive analysis of ascomycete's genomes, sequences for lipase genes were identified and subjected to further bioinformatics analysis. The 3D structures of the identified enzymes were predicted, and molecular docking simulations were carried out for analyzing the interaction between lipase and six different microplastics including polycarbonate (PC) and polyethylene terephthalate (PET). The results of molecular docking revealed the key residues that are involved in the protein-ligand interaction and also provided and insight into the binding affinities. The finding of this study showcases the potential of fungal lipases for microplastic degradation, especially with reference to most abundant ones like PC and PET. Further experimental investigation is required for the validation of these results for real world applications.
Page(s):
137-137
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
pollution
,
Lipase
,
docking
,
Microplastics
,
fungal enzymes
,
polyethylene terephthalate
References:
References are not available for this document.
Citations
Citations are not available for this document.