Abstract:
An overall incidence of 14.08% of Pythium damping off was recorded on chili in the Pothwar region of the Punjab province of Pakistan. Attock showed the maximum incidence of 19.86% followed by Rawalpindi and Chakwal districts having 16.22% and 10.22% disease incidences respectively. Jhelum had the minimum disease incidence of 10.05%. Disease severity was the maximum in district Attock (11.03%) followed by Rawalpindi (9.11%) and was the minimum in Chakwal (5.42%) followed by Jhelum (5.67%). In case of Phytophthora blight, the overall incidence on chili was found to be 14.46%. Incidence was found to be higher in Rawalpindi (18.44%) followed by Attock, Jhelum and Chakwal, where the incidences of the disease were 16.52%, 12.66% and 10.23% respectively. Disease severity was the maximum in Rawalpindi (6.63%) followed by Attock (6.12%) and was the minimum in Jhelum (3.69%) followed by Chakwal (3.54%). In total, 39 isolates of Pythium from chili were recovered in Pothwar. All the isolates showed variations in different morphological parameters and were confirmed to be Pythium aphanidermatum and P. ultimum. Out of 39 isolates of Pythium spp. from chili, 8% were highly virulent, 18% virulent, 36% moderately virulent and 38% were nonpathogenic. In case of Phytophthora, a total of 36 isolates from chili were recovered. All the isolates showed variations in various morphological characteristics and were recognized as Phytophthora infestans and P. capsici. All the isolates of Pythium and Phytophthora spp. produced bands of 800 bp with ITS1 and ITS universal primers respectively and were confirmed to be Pythium and Phytophthora spp. The phylogenetic analysis showed close relationship among all the isolates. The finding of these studies will be helpful for the farmers in designing control strategies for these two pathogens accordingly. The information will also be useful for the breeders to develop resistant varieties of chili against the most virulent isolates of the pathogen.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Phytophthora
,
Pothwar region
,
chili
,
Pythium