Trichoderma Aspereullm Accc30536 Inoculation Differently Regulates the Time-course Expression of Five Indole-3-acetic Acid Amido Synthetase Genes and the Levels of IAA, SA and JA in Populus davidiana × P. Alba Var. Pyramidalis. | [Pakistan Journal of Botany • 2019]

Author(s):

1. TONGTONG ZHAI: College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China

2. YUEFENG WANG: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

3. Abdul Majeed Baloch: College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China

4. Abdul Wahid Baloch: Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam, Pakistan

5. ZhaoYing Liu: College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China

6. CHUANYING JIANG: College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China

7. RONGSHU ZHANGR: College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China

Abstract:

Some indole-3-acetic acid amido synthetases (GH3 proteins) were confirmed to catalyze the connection between IAA, SA or JA with amino acid to regulate hormone dynamic balances in promoting plant growth; they can also improve plants' resistance to stress through activating SA, JA and ethylene signaling pathways. Trichoderma spp. are widely-known beneficial biocontrol agents. However, the expression pattern of plant GH3 genes induced by Trichoderma remained unexplored. In this study, five GH3 genes in Populus davidiana × P. alba var. pyramidalis (named PodaGH3-1, PodaGH3-2, PodaGH3-5, PodaGH3-9 and PodaGH3-10) were cloned. Their characteristic analysis showed that the five PodaGH3s genes were highly conservative and closely related at the residues linked to ATP binding with IAA or JA, and their expression levels changed obviously under Trichoderma inoculation. Moreover, IAA, JA and SA contents showed a fluctuating yet increasing trend during the 72 hours when poplar seedling rhizosphere was colonized by Trichoderma. In summary, all the five PodaGH3s could be activated by the interaction between biotrophic Trichoderma and poplar seedlings.

Page(s): 689-697

DOI: 10.30848/PJB2019-2(34)

Published: Journal: Pakistan Journal of Botany, Volume: 51, Issue: 2, Year: 2019

Keywords:

RTqPCR , Trichoderma aspereullm , indole3acetic acid amido synthetase , Populus davidiana × P alba var pyramidalis

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