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Effects of Combretin A in Murine Model of Nicotine Dependence
Author(s):
1. Naeem Ur Rehman: Department of Pharmacy, Gomal University Dera Ismail Khan, Khyber Pakhtoonkhwa, 29220 Pakistan
2. Muhammad Sona Khan: Pharmacy Services Department, Ayub Teaching Hospital Abbottabad Khyber Pakhtoonkhwa, 22060, Pakistan
3. Ahmed Tokhi: Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Khyber Pakhtoonkhwa, 22060, Pakistan
4. Mehreen Arif: Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Khyber Pakhtoonkhwa, 22060, Pakistan
5. Khalid Rauf: Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Khyber Pakhtoonkhwa, 22060, Pakistan
Abstract:
Nicotine dependence is the single largest preventable cause of death and disease in the world. The World Health Organization estimates that in 2015 over 1.1 billion individuals used some form of tobacco. Constant use of nicotine offers different health related issues, for example, malignant growth, lung malfunction, coronary illness and furthermore generalized weakness to various kinds of irresistible infections and mild to moderate neurological problems like anxiety, hyperalgesia and depression. Combretin A is cycloartane type triterpenes extracted from fraction of the MeOH extract of the leaves of C. fragrans. This plant has been researched and appeared to have antifungal, antibacterial, analgesic and anti-proliferative activity. Current study was designed to evaluate the potential effects of Combretin A in nicotine withdrawal induced abstinence/withdrawal signs, locomotor activity, withdrawal induced hyperalgesia and withdrawal induced depression-like behavior in mice via withdrawal scale, locomotor test, thermal/mechanical test, and tail suspension. For induction nicotine 2mg/kg/day was given in four divided doses, intraperitoneally for 14 consecutive days except normal saline group. Combretin A single dose of 25mg/kg and 50mg/kg was administered via oral gavage and reading was noted for each set of experiment. Our study depicted that administration of Combretin A at both doses (25 and 50 mg/kg) did not reduce overall nicotine withdrawal abstinence signs, but showed significance results at 50mg/kg after 24 and 48 hours of withdrawal in open field parameters. Combretin A at both doses (25 and 50 mg/kg) significantly reverse nicotine withdrawal induced thermal hyperalgesia. In mechanical hyperalgesia no statistical significance was observed but showed partially amelioration of depression like behavior at 50mg/kg, which was statistically non-significant. This partial effect may be attributed to its anti-inflammatory, anti-oxidant, and analgesic actions as reported in literature. In conclusion, it can be summarized that due to its diverse properties, Combretin A may be a potential therapeutic remedy in the treatments of pain models associated with addiction.
Page(s): 57-57
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Depression , Nicotine , Nicotine , dependence , Withdrawal
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