Abstract:
Diabetes and inflammatory disorders are two devastating conditions that a significant proportion of the world’s population is suffering from. The concept of inflammation is the swelling brought on by an injury, pyrexia is characterized by a rise in body temperature, diabetes is defined as an increase in blood glucose level, and pain is an unpleasant experience sometimes associated with damage. The adverse consequences of conventional medications used to treat these conditions led people to seek out safer alternatives. The medicinal plant has traditionally been used to make folk remedies, and its popularity is growing as a result of its affordability, safety, and lack of side effects when compared to conventional drugs. The study aimed to assess the various pharmacological activities of Cyamopsis tetragonoloba i.e. in vivo anti-inflammatory, antipyretic, anti-diabetic, and analgesic activities. Furthermore, this study comprises an eco-friendly approach for the production of Zinc oxide Nanoparticles by using C. tetragonoloba fruit extract as a reducing and stabilizing agent. Phytochemical extraction was carried out using methanol, dichloromethane, ethyl acetate, and n-hexane. Inflammation, pyrexia, and diabetes were induced in experimental animals using 1% carrageenan, 20% Brewer yeast, and alloxan respectively. The pain was induced by two different means firstly by placing animals on a hot plate and then by immersing the tail of animals in hot water. Extracts at the dose level of 300 and 500mg/kg BW were orally administered in rats to evaluate the in-vivo anti-inflammatory, antipyretic, anti-diabetic, and analgesic potential of the biologically active compounds. Extracts' anti-inflammatory, antipyretic, and analgesic effects were compared to those of the widely used anti-inflammatory, antipyretic, and anti-nociceptive drugs (Diclofenac and paracetamol), while their anti-diabetic effects were studied in comparison to glibenclamide. The percentage peak abundance and the quantity of phytochemicals that were extracted from the C. tetragonoloba extracts in MtOH, DCM, E.A, n-Hex solvents differed significantly. The overall response produced by each extract used in the study was dose-dependent. The anti-inflammatory activity of the extracts was linked with a decrease in paw volume compared to the control groups. The maximum dose of MtOH extract (500 mg/kg) reduced paw edema from 5.38±1.56 to 3.77±0.006 which is considered highly significant as the edema inhibition by Diclofenac was from 6.18±0.006 to 3.78± 0.015. Likewise, the MtOH extract (500 mg/kg) showed greater efficacy in its antipyretic action by lowering body temperature from 103.71±0.71 to 100.36±0.54, which was almost equivalent to the temperature reduced by the standard drug i.e. 103.89±0.058 to 98.66±0.12. The anti-diabetic potential was confirmed when the decrease in blood glucose level from MtOH extract was compared with the standard drug. The highest dose of MtOH extract (500mg/kg) decreased the blood glucose level from 216.2±0.03 to 143±1.03 mg/dL, and glibenclamide decreased it from 202.71±1.04 to 122±0.008. In the case of an analgesic assay, the reaction time was significantly increased by MtOH extract (500mg/kg). UV-Vis spectroscopy revealed a sharp peak between 360-380nm which confirmed ZnO-mediated NPs, and the presence of various functional groups was confirmed by Fourier transform infrared. In a nutshell, this study confirmed the role of C. tetragonoloba extract in disease management. The outcomes of each trial suggested that MtOH extracts of C. tetragonoloba possess efficient anti-inflammatory, anti-pyretic, anti-diabetic, and analgesic potential. The overall trend of extracts was ranked as MtOH>DCM>E.A>n-Hex. This may be due to presence of surplus amount of phytochemicals in MtOH extract of C. tetragonoloba. The presence of ZnO NPs was confirmed by UV-Vis spectroscopy peaks and functional group present were confirmed by FT-IR analysis. Further investigation is required to validate the findings.
Page(s):
96-96
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Nanoparticles
,
Green synthesis
,
Cyamopsis tetragonoloba
,
antiinflammation effect