Pakistan Science Abstracts
Article details & metrics
No Detail Found!!
The Cfaos and Cfaoc Genes Related to Flower Fragrance Biosynthesis in Cymbidium faberi Could Confer Drought Tolerance to Transgenic Tomatoes.
Author(s):
1. Yin Zhou: Center of Applied Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P. R. China: College of Bioscience and Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P.R. China
2. Li Chen: Center of Applied Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P. R. China: College of Bioscience and Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P.R. China
3. Yanqin Xu: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, P.R. China
4. Yuting Wang: Center of Applied Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P. R. China: College of Bioscience and Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P.R. China
5. Songtai Wang: Center of Applied Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P. R. China: College of Bioscience and Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P.R. China
6. Xiaoxia Ge: Center of Applied Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P. R. China: College of Bioscience and Biotechnology, Wuhan Institute of Bioengineering, Wuhan 430415, P.R. China
Abstract:
Cymbidium faberi is one of the longest cultivated oriental orchids. It has a strong flower fragrance, which is an economically important trait in oriental orchids. However, the molecular mechanism of biosynthesis and regulation of flower fragrance in C. faberi remains unclear. Methyl jasmonate (MeJA), one of the main components of flower fragrance in C. faberi, plays multiple roles, including insect attraction, as well as the mediation of anti-microbial and stress tolerance. Allene oxide synthase (AOS) and allene oxide cyclase (AOC) are crucial enzymes in the MeJA biosynthetic pathway. In this study, we cloned the CfAOS and CfAOC genes from C. faberi and overexpressed them individually in transgenic tomato plants. Whereas no increase in MeJA could be detected in the transformants, the expression level of the jasmonate-related SlLOX gene was significantly upregulated. Moreover, the expression levels of MeJA related genes, like SlLOX, SlAOS, SlAOC, SlOPR3 and SlJMT in tomato transformants were increased in drought stress treatment. These results not only provide useful information for uncovering the biosynthesis mechanism of flower fragrance in C. faberi, but offer an example of unexpected wider applications in terms of stress resistance which can arise from basic research of flower fragrance.
Page(s): 883-892
Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 4, Year: 2018
Keywords:
Keywords are not available for this article.
References:
[1] Agrawal , G.K.,R.Rakwal,N.S.Jwa,K.S.Han,V.P.Agrawal, 2002.Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase,Plant 40 771 -782
[2] Baldassarre , V.,G.Cabassi,N.D.Spadafora,A.Aprile,C.T.Müller,H.J.Rogers,A.Ferrante, 2015.Wounding tomato fruit elicits ripening-stage specific changes in gene expression and production of volatile compounds,J. Exp. Bot. 66 1511 -1526
[3] Boatright , J.,F.Negre,X.Chen,C.M.Kish,B.Wood,G.Peel,I.Orlova,D.Gang,D.Rhodes,N.Dudareva, 2004.Understanding in vivo benzenoid metabolism in petunia petal tissue,Plant 135 1993 -2011
[4] Cai , J.,X.Liu,K.Vanneste,S.Proost,W.C.Tsai,K.W.Liu,L.J.Chen,Y.He,Q.Xu,C.Bian,Z.Zheng,F.Sun,Y.Y.Hsiao,Z.J.Pan,C.C.Hsu,Y.P.Yang,Y.C.Hsu,Y.C.Chuang,A.Dievart,J.F.Dufayard,X.Xu,J.Y.Wang,J.Wang,X.J.Xiao,X.M.Zhao,R.Du,G.Q.Zhang,M.Wang,Y.Y.Su,G.C.Xie,G.H.Liu,L.Q.Li,L.Q.Huang,Y.B.Luo,H.H.Chen,Y.Van de Peerand Z.J. Liu, 2015.The genome sequence of the orchid Phalaenopsis equestris,Nat. Genet 47 65 -72
[5] Chang , Z.,L.Li,Z.Pan,X.Wang, 2008.Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum,Acta Crystallogr. Sect. F Struct. Biol. Cryst 64 668 -670
[6] Croteau , R.,F.Karp,P.M.Muller, 1991.Origin of natural odorants,In: Perfumes. Art, Science and Technology 101 -126
[7] Dudareva , N.,E.Pichersky,J.Gershenzon, 2004.Biochemistry of plant volatiles,Plant 135 1893 -1902
[8] Dudareva , N.,L.M.Murfitt,C.J.Mann,N.Gorenstein,N.Kolosova,C.M.Kish,C.Bonham,K.Wood, 2000.Developmental regulation of methyl benzoate biosynthesis and emission in snapdragon flowers,Plant Cell 12 949 -961
[9] Dudareva , N.,J.C. D'Auria , K.H.,Nam , R.A.,Raguso and E.,Pichersky, 1998.Benzylalcohol acetyltransferase-an enzyme involved in floral scent production in Clarkia breweri,Plant 14 297 -304
[10] Flament , I., C.,Debonneville and A.,Furrer, 1993.Volatile constituents of roses: Characterization of cultivars based on the headspace analysis of living flower emissions,J. Biol. Chem 278 48805 -48814
[11] Froehlich , J.E.,A.Itoh,G.A.Howe, 2001.Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope,Plant 125 306 -317
[12] Gohain , B.,P.Bhorali,N.Agarwal,L.P.Bhuyan,A.Rahman,K.Sakata,M.Mizutani,B.Shimizu,R.Ravindranath,M.C.Kalita,M.Hazarika and S. Das, 2012.Understanding Darjeeling tea flavour on a molecular basis,Plant Mol. Biol 78 577 -597
[13] Goossens , J.,P.Fernández-Calvo,F.Schweizer,A.Goossens, 2016.Jasmonates: signal transduction components and their roles in environmental stress responses,Plant Mol. Biol 91 673 -689
[14] Harms , K.,R.Atzonr,A.Barsh,H.Kuhn,C.Wasternack,L.Willmitzer,H.Pena-Cortes, 1995.Expression of a flax allene oxide synthase cDNA leads to increased endogenous jasmonic acid (JA) levels in Transgenic potato plants but not to a corresponding activation of JAresponding genes,Plant Cell 7 1645 -1654
[15] Hause , B.,O.Miersch,H.Maucher,R.Kramell,J.Ziegler,C.Wasternack, 2000.Tissue-specific oxylipin signature of tomato flowers-allene oxide cyclase is highly expressed in distinct flower organs and vascular bundles,Plant 24 113 -126
[16] Hossain , M.M.,M.Sharma,J.A., 2010.Seed germination and tissue culture of Cymbidium giganteum Wall,ex Lindl. Sci. Hortic 123 479 -487
[17] Holsters , M.,D. de Waele , A.,Depicker , E. Messens, M. Van,Montagu and J.,Schell, 1978.Transfection and transformation of Agrobacterium tumefaciens,Mol. Gen. Genet 163 181 -187
[18] Howe , G.A.,G.I.Lee,A.Itoh,L.Li,A.E.DeRocher, 2000.Cytochrome P450-dependent metabolism of oxylipins in tomato. Cloning and expression of allene oxide synthase and fatty acid hydroperoxide lyase,Plant 123 711 -724
[19] Huang , M.,C.Ma,R.Yu,L.Mu,J.Hou,Y.Yu,Y.Fan, 2015.Concurrent changes in methyl jasmonate emission and the expression of its biosynthesis-related genes in Cymbidium ensifolium flowers,Physiol. Plant 153 503 -512
[20] Huber , F.K.,R.Kaiser,W.Sauter,F.P.Schiestl, 2005.Floral scent emission and pollinator attraction in two species of Gymnadenia (Orchidaceae),Oecologia 142 564 -575
[21] Laudert , D.,E.W.Weiler, 1998.Allene oxide symhase: a major control point in Arabidopsis thaliana octadecanoid signaling,Plant 15 675 -684
[22] Laudert , D.,F.Schaller,E.W.Weiler, 2000.Transgenic Nicotiana tabacum and Arabidopsis thaliana plants overexpressing allene oxide synthase,Planta 211 163 -165
[23] Lee , D.S.,P.Nioche,M.Hamberg,C.S.Raman, 2008.Structural insights into the evolutionary paths of oxylipin biosynthetic enzymes,Nature 455 363 -368
[24] Maucher , H.,I.Stenzel,O.Miersch,N.Stein,M.Prasad,P.Schweizer,C.Dorer,B.Hause,C.Wasternack, 2004.-cloning and organspecific expression,Phytochemistry 65 801 -811
[25] Mei , C.,M. Qi , G.,Sheng and Y.,Yang, 2006.Inducible overexpression of a rice AOS gene increase the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection,Mol. Plant 19 1127 -1137
[26] Miersch , O.,H.Weichert,I.Stenzel,B.Hause,H.Maucher,I.Feussner,C.Wasternack, 2004.Constitutive overexpression of allene oxide cyclase in tomato (Lycopersicon esculentum cv Lukullus) elevates levels of some jasmonates and octadecanoids in flower organs but not in leaves,Phytochemistry 65 847 -856
[27] Niu , Y.,P.Figueroa,J.Browse,Exp, 2011.Characterization of JAZinteracting bHLH transcription factors that regulate jasmonate responses in Arabidopsis, 62 2143 -2154
[28] Omata , A,S.Nakamura,K.Yomogida,K.Moriai,Y.Ichikawa,I. Watanabe, 1990.Volatile components of TO-YO-RAN flowers (Cymbidium faberi and Cymbidium virescens),Agric. Biol. Chem 54 1029 -1033
[29] Pan , Z.,F.Durst,D.Werck-Reichhart,H.W.Gardner,B.Camara,K.Cornish,R.A.Backhaus, 1995.The major protein of guayule rubber particles is a cytochrome p450 characterization based on cDNA cloning and spectroscopic analysis of the solubilized enzyme and its reaction products,J. Biol. Chem 270 8487 -8494
[30] Park , J.,R.Halitschke,H.B.Kim,I.T.Baldwin,K.A.Feldmann,R.Feyereisen, 2002.A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal tranduction in Arabidopsis due to a block in jasmonic acid biosynthesis,Plant 31 1 -12
[31] Peng , H.M., 2009.Study on the Volatile, Characteristic Floral Fragrance Components of Chinese Cymbidium. Chinese academy of forestry, -
[32] Peng , X.,Y.Hu,X.Tang,P.Zhou,X.Deng,H.Wang,Z.Guo, 2012.Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice,Planta 236 1485 -1498
[33] Riemann , M.,K.Haga,T.Shimizu,K.Okada,S.Ando,S.Mochizuki,Y.Nishizawa,P.Nick,M.Yano,M.Takano,H.Yamane,M.Lino, 2013.Identification of rice Allene Oxide Cyclase mutants and the function of jasmonate for defence against Magnaporthe oryzae,Plant 74 226 -238
[34] Rodriguez-Saona , C.,L.Parra,A.Quiroz,R.Isaacs, 2011.Variation in highbush blueberry floral volatile profiles as a function of pollination status, cultivar, time of day and flower part: implications for flower visitation by bees, 107 1377 -1390
[35] Seo , H.S.,J.T.Song,J.J.Cheong,Y.H.Lee,Y.W.Lee,I. Hwang,J.S.Lee,Y.D.Choi, 2001.Jasmonic acid carboxyl methyltransferase: a key enzyme for jasmonate-regulated plant responses,Proc. Natl. Acad. Sci. USA 98 4788 -4793
[36] Sivasankar , S.,B.Sheldrick,S.J.Rothstein, 2000.Expression of allene oxide synthase determines defense gene activation in tomato,Plant 122 1335 -1342
[37] Song,C.D. Funk and A.R.,Brash, 1993.Molecular cloning of an allene oxide synthase: A cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides,Proc. Natl. Acad. Sci. USA 90 8519 -8523
[38] Staswick , P., 1999.Sequence of an allene oxide synthase cDNA from Arabidopsis (Accession No. AF172727),Plant Physiol 121 312 -
[39] Stenzel , I.,M.Otto,C.Delker,N.Kirmse,D.Schmidt,O.Miersch,B.Hause,C.Wasternack, 2012.ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thaliana: tissue- and organspecific promoter activities and in vivo heteromerization,J. Exp. Bot. 63 6125 -6138
[40] Stenzel , I.,B.Hause,O.Miersch,H.Maucher,H.Weichert,J.Ziegler,I.Feussner,C.Wasternack, .Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana,Plant Mol. Biol 51 895 -911
[41] Stenzel , I.,B.Hause,H.Maucher,A.Pitzschke,O.Miersch,J.Ziegler,C.A.Ryan,C.Wasternack, .Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato-amplification in wound signaling,Plant 33 577 -589
[42] Wang , C.,S.Avdiushko,D.F.Hidebrand, 1999.Overexpression of a cytoplasm-localized allene oxide synthase promotes the woundinduced accumulation of jasmonic acid in transgenic tobacco,Plant Mol. Biol 40 783 -793
[43] Wu , J.J.,Q.Wu,J.Gai,D.Yu, 2008.Constitutive overexpression of AOS-like gene from soybean enhanced tolerance to insect attack in transgenic tobacco, 30 1693 -1698
[44] Yan , L.,Q.Zhai,J.Wei,S.Li,B.Wang,T.Huang,M.Du,J.Sun,L.Kang,C.B.Li,C.Li, 2013.Role of tomato Lipoxygenase D in woundinduced jasmonate biosynthesis and plant immunity to insect herbivores,PLoS Genet 9 e1003964 -
[45] Zhang , H. and J.,Memelink, 2009.,Natural Products. Springer Science & Business Media (DOI: 10.1007/978-0-387- 85498-4_8) -
[46] Zhang , L.Y., M.Y.,Bai , J.,Wu , J.Y.,Zhu , H.,Wang , Z.,Zhang , W.,Wang , Y.,Sun , J.,Zhao , X.,Sun , H.,Yang , Y.,Xu , S.H.,Kim , S.,Fujioka , W.H.,Lin , K.,Chong , T.,Lu and Z.Y.,Wang, 2009.Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and Arabidopsis,Plant Cell 21 3767 -3780
[47] Zhao , Y.,W.Dong,N.Zhang,X.Ai,M.Wang,Z.Huang,L.Xiao,G.Xia, 2014.A wheat allene oxide cyclase gene enhances salinity tolerance via jasmonate signaling,Plant 164 1068 -1076
[48] Zhou , Y., S.,Wang , K.,Dong , H.,Hong and X.,Ge, 2015.Cloning and expression analysis of allene oxide cyclase gene (CfAOC) in Cymbidium faberi,North. Hortic. 14 84 -89
[49] Zhou , Y.,Y.Yau,D.W.Ow,Y.Wang, 2012.ParA-MRS recombination systems,Plant Biotechnol. Rep 6 225 -232
[50] Ziegler , J.,M.Keinanen,I.T.Baldwin, 2001.Herbivore-induced allene oxide synthase transcripts and jasmonic acid in Nicotiana attenuate,Phytochemistry 58 729 -738
Citations
Citations are not available for this document.
0

Citations

0

Downloads

6

Views