Functional Characterization of Rice Genes Controlling Meristems Identity Determination using CRISPR Cas9 Genome Editing Tool | [Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 • 2023]

Author(s):

1. Israr Ud Din: Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy; Institute of Biotechnology and Genetic Engineering (IBGE), University of Agriculture, Peshawar 25130, Pakistan

2. Emanuela Franchini: Dipartimento di Bioscienze, Università degli Studi di Milano,Via Celoria 26, 20133, Milano, Italy

3. Veronica M. Beretta: Dipartimento di Bioscienze, Università degli Studi di Milano,Via Celoria 26, 20133, Milano, Italy

4. Elia Lacchinia: VIB Center for Plant Systems Biology, Technologiepark 71, B-9052 Ghent, 14 Belgium

5. Lisa Van den Broeck: Plant and Microbial Biology Department, North Carolina State University, Raleigh, NC 27695

6. Rosangela Sozzani: Plant and Microbial Biology Department, North Carolina State University, Raleigh, NC 27695

7. Gregorio Orozco-Arroyo: Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy

8. Hélène Adam: 5DIADE, University of Montpellier, IRD, CIRAD, Montpellier, France

9. Stefan Jouannic: DIADE, University of Montpellier, IRD, CIRAD, Montpellier, France

10. Veronica Gregis: Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy

11. Martin M. Kater: Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy

Abstract:

The architecture of the rice inflorescence is an important determinant of seed yield. The length of the inflorescence and the number of branches are among the key factors determining the amount of spikelets, and thus seeds, that will develop. Especially the timing of the identity transition from indeterminate branch meristem to determinate spikelet meristem regulates the complexity of the inflorescence. In this context, the ALOG gene TAWAWA1 (TAW1) has been shown to delay the transition to determinate spikelet development in rice. Recently, by combining precise laser microdissection of inflorescence meristems with RNA-seq we observed that two ALOG genes, Oryza sativa OsG1-like 1 (OsG1L1) and OsG1L2, have an expression profile similar to TAW1. Here we report that osg1l1 and osg1l2 loss-of-function CRISPR mutants have similar phenotypes as the taw1 mutant, suggesting that these genes might act on related pathways during inflorescence development. Transcriptome analysis of the osg1l2 mutant suggested interactions of OsG1L2 with other known inflorescence architecture regulators and the datasets were also used for the construction of a gene regulatory network (GRN) proposing interactions between genes potentially involved in controlling inflorescence development in rice. The spatio-temporal expression profiling and phenotypical analysis of CRISPR loss-of-function mutants of the homeodomain-leucine zipper transcription factor gene OsHOX14 suggest that the proposed GRN indeed serves as a valuable resource for the identification of new players involved in rice inflorescence development. In conclusion, OsG1L1 and OsG1L2 control panicle architecture through delaying the transition from indeterminate branch- to determinate spikelet-meristem identity.

Page(s): 28-28

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

meristem , OsHOX14 , OsG1L2 , ALOG , inflorescence architecture

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