Abstract:
Integrative taxonomy and population study methods are being used widely in species and their population determination and delimitation; it is quite comprehensive approach to find out their intra and interspecific relationships. Along with morphometric analyses, amylase, creatinine kinase isozymes and native Coomassie marker were used to investigate the genetic diversity and discrimination among population structure of genus Metapenaeus sp. A total of 117 individual shrimps were collected from four locations. The mean size was significantly varied among the four populations. From 1st segment, muscles were taken for analysis and native PAGE were used for protein dissolution and a total of 25 loci were observed. 48% loci were found polymorphic such as in CA-1 allele were resolved at four loci (A=0.444, B=0.111, C= 0.278 and D= 0.167) while CAT-1 and CK-2 represented A and B equally. AMY-5and CK -5 showed frequency of allele A and C as 0.972 and 0.028. These were found to be significantly different CA-2, CA-3, AMY-1, AMY-2, CAT-2, CK-1 and CK-4. Na, Ne, and I were found to be 1.680 ± 0.852, 1.376 ± 0.588 and 0.295 ± 0.395 respectively, observed and expected heterozygosity was 0.264±0.374 and 0.184 ± 0.245 which indicated that the four populations of Metapenaeus sp. possessed a rich genetic diversity. The deviation from Hardy-Weinberg equilibrium (P<0.05) was observed as Fst and Nm were 0.249 and 0.752 respectively. The results were based on the number of alleles as the main indication of genetic differentiation. The species of Korangi, Ketibander and Karachi fish harbor were found to be falling in the same cluster while Sonmiani population was mostly acquiring different cluster.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Native Coomassie
,
Isozymes
,
genetic diversity
,
Metapenaeus
,
Creatinine kinase
,
amylase