Abstract:
Genetic variation in cotton for salinity tolerance is a prerequisite for developing genetically improved cultivars. The objective of the study was to assess the genetic variation for salt tolerance in mutated M 3 population of cotton developed by gamma irradiation and EMS. A screening experiment was conducted for 12 mutated and one normal/untreated genotype in a hydroponics system under controlled conditions. The three salinity levels used were control, 150 mM and 300 mM. Three leaf seedlings were transplanted to hydroponics having half-strength Hoagland solution at control, 150 mM, and 300 mM NaCl salinity levels. Salinity induced significant variation in root and shoot lengths, fresh root and shoot weight, dry root and shoot weight, root shoot ratio, survival rate, protein contents, and a-amylase activity were observed. Genotypes 9Ac, 47Ab, 1Aa, 45Aa, and PB-899 were observed highest tolerant genotypes with a better performance at 150 mM salinity level. However, at 300 mM salinity level best genotypes included were 57ab, 47Ab, 1Aa, and 54Aa. On the other hand, genotypes 7Bb, 51Aa, and 57ab showed poor performance or susceptible response across the 150 mM saline environment. Genotypes 9Ba, 45Aa, 9Ac and PB-899 were susceptible at 300 mM salinity. Thus, the genotypes 57ab, 47Ab and 1Aa were found tolerant to adoption of salt stress and can be used as a source in crop improvement program.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
cotton
,
mutagenesis
,
Salinity tolerance
,
TILLING population
,
Hydroponics technique
,
biochemical response