Abstract:
Schizophrenia is a complex neuropsychiatric disorder with a multifactorial etiology, involving both genetic and environmental factors. The DRD2 geneplays a crucial role in various neuropsychiatric disorders and drug addiction. The dopamine D2 receptor gene (DRD2) has been extensively studied due to its association with schizophrenia. Microsatellite polymorphisms within the DRD2 gene have been proposed as potential genetic markers for susceptibility to schizophrenia. In this study, we aimed to investigate the role of microsatellite polymorphisms in the DRD2 gene in schizophrenia susceptibility using polymerase chain reaction (PCR) amplification and computational analysis. Genomic DNA samples were collected from individuals diagnosed with schizophrenia and healthy controls. Primers were designed to specifically amplify regions containing microsatellite repeats within the DRD2 gene. PCR amplification was performed, and the resulting DNA fragments were analyzed using gel electrophoresis to detect length variations indicative of microsatellite polymorphisms. The PCR-based genotyping approach successfully identified microsatellite polymorphisms in the DRD2 gene. We observed variations in the sizes of amplified DNA fragments, indicating the presence of different alleles for the identified microsatellite markers. Furthermorewe conducted a comprehensive computational analysis of the DRD2 gene, utilizing various bioinformatics tools and databasessuch as, STRING's, KEGG, Reactome and BioGrid databases that may provide insights into the biological processes underlying schizophrenia. In conclusion, our study successfully identified microsatellite polymorphisms within the DRD2 gene associated with schizophrenia using PCR amplification. These findings contribute to the growing body of evidence linking genetic variations in the DRD2 gene to schizophrenia susceptibility. The computational analysis of the DRD2 gene provides a comprehensive overview of its structure, function, regulation, and genetic variations. Further investigation of these microsatellite markers in larger cohorts are warranted to elucidate their precise role in the pathogenesis of schizophrenia and to explore their potential utility as diagnostic markers in clinical settings.
Page(s):
250-250
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
PCR
,
Schizophrenia
,
microsatellite polymorphism
,
computational anaylsis
,
DRD2