Genome size variations among different date palm cultivars (phoenix dactylifera L.) by flow cytometry | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Tahira Jatt: Department of Botany, Shah Abdul Latif University, Khairpur, Sindh, Pakistan

2. Ray Ming: Department of Plant Biology, University of Illinois, Urbana–Champaign

3. Moon-Sub Lee: Department of Crop Sciences, University of Illinois, Urbana-Champaign, IL.61802, USA

4. A. Lane Rayburn: Department of Crop Sciences, University of Illinois, Urbana-Champaign, IL.61802, USA

5. Ghulam Sarwar Markhand: Department of Botany, Shah Abdul Latif University, Khairpur, Sindh, Pakistan

6. Mushtaque Ahmed Jatoi: Department of Botany, Shah Abdul Latif University, Khairpur, Sindh, Pakistan

7. Abdul Aziz Mirani: Department of Botany, Shah Abdul Latif University, Khairpur, Sindh, Pakistan

Abstract:

Khairpur is a city in Sindh, province of Pakistan, known as the biodiversity center of date palm. Date palm (Phoenix dactylifera L.) is a monocotyledonous, dioecious, diploid species, has uncertain ploidy levels having 14 (2n=28) to 18 (2n=36) pairs of chromosomes because of scarcity of cytogenetic knowledge. The accurate amount of nuclear DNA content is extremely important to understand the hereditary and evolutionary aspect of organisms. Flow cytometry is an easy, rapid, accurate and convenient tool for estimating plant genome size, ploidy level and assessing DNA content. Therefore, the present study was conducted with aim to estimate the genomic size (C value) of fifty commercial date palm cultivars along with four wildly grown date palm plants by flow cytometry using Propidium Iodide (PI) as the fluorescent dye and corn (Zea maiz L.) genome size as internal reference. Genome size of cultivars significantly varied and ranged from 1.595 pg 2C-1 to 1.847 pg 2C-1. Size of nuclei significantly varied from cultivar to cultivar and appeared independent to their genomic size. Five exotic cultivars (Amber, Sugae, Medjool, Safawi and Ajwa) grown in Pakistan were compared of their origin.

Page(s): 182-182

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

Phoenix dactylifera L , Flow cytometry , genome , propidium iodide PI

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