Volatile constituents and in vitro activity of Syzygium aromaticum flower buds (clove) against human cancer cell lines | [Pakistan Journal of Pharmaceutical Sciences • 2020]

Author(s):

1. Sabira Begum: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan

2. Syed Nawazish Ali: Department of Chemistry University of Karachi,Karachi,Pakistan

3. Ahsana Dar Farooq: Hamdard Al-Majeed College of Eastern Medicine Hamdard University,Karachi,Pakistan

4. Faheema Siddiqui: Faculty of Pharmaceutical Sciences, Dow College of Pharmacy, Dow University of Health Sciences,Karachi,Pakistan

5. Bina S Siddiqui: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi,Pakistan

6. Anjum Ayub: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan; NED University of Engineering & Technology, Karachi, Pakistan

7. Mashhad Fatima: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi,Pakistan

8. Sara: HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan; Department of Chemistry University of Karachi, Karachi, Pakistan

Abstract:

The methanolic extract (SA-EXT) of Syzygium aromaticum flower buds and its fractions tested against three human cancer cell lines viz uterine cervix (HeLa), breast (MCF-7) and lung NCI (H-460) using sulforhodamine-B assay. The ethyl acetate soluble sub fraction (SA-EAS) was active only against HeLa cells with GI50value of 36± 3.4µg/mL. The most active sub-fraction (SA-PES-Fr-2) showed growth inhibition (GI50: 36, 50 and 68µg/ml against MCF-7, HeLa and NCI-H-460 cancer cell lines, respectively) with cytotoxic effect LC50= 88 ± 3.4 µg/mL against HeLa and LC50=86 ± 2.8 µg/mL against MCF-7. The most active sub-fraction (SA-PES-Fr-2) analyzed by GC and GC-MS techniques revealed that eugenol was most abundant (85.34%) along with minor constituents. Thus it can be concluded that growth inhibitory and cytotoxic effect residing in Syzygium aromaticum flower buds (clove) is more likely due to eugenol.

Page(s): 2659-2665

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 33, Issue: 6, Year: 2020

Keywords:

cytotoxicity , GCMS , Syzygium aromaticum , Growth inhibition , Eugenol , sulforhodamineB

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