A STUDY ON BIOCHEMICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF HONEY FROM DERA BUGTI DISTRICT OF BALOCHISTAN | [Journal of Animal and Plant sciences • 2022]

Author(s):

1. S. Ambreen: Institute of Biochemistry, University of Balochistan,Quetta,Pakistan

2. Z. Mehmood: Institute of Biochemistry, University of Balochistan,Quetta,Pakistan

3. K. Mehmood: Institute of Biochemistry, University of Balochistan,Quetta,Pakistan

4. A. Sajjad: Institute of Biochemistry, University of Balochistan,Quetta,Pakistan

5. A. Mushtaq: Department of Chemistry, Sardar Bahadur Khan Women Usniversity Quetta,Quetta,Pakistan

6. S. Rizwan: Department of Chemistry, Sardar Bahadur Khan Women Usniversity Quetta,Quetta,Pakistan

7. U. Jabeen: Department of Chemistry, Sardar Bahadur Khan Women Usniversity Quetta,Quetta,Pakistan

8. F. Bashir: Department of Chemistry, Sardar Bahadur Khan Women Usniversity Quetta,Quetta,Pakistan

9. F. Behlil: Department of Chemistry, Sardar Bahadur Khan Women Usniversity Quetta,Quetta,Pakistan

Abstract:

20 honey samples from district, Balochistan (Pakistan) were studied for their physiochemical, biochemical and antioxidant potential. During the study, pH, ash content, moisture, total fat, sugar content, reducing and non-reducing sugars, vitamin C, Vitamin A, flavonoids, polyphenols and % DPPH scavenging activity were monitored. The pH of the samples was between4.0 - 5.0, having mean±SD values of 4.5 ± 0.1. The moisture and ash content had an average value of 17.4 ± 0.7 g/100g and 0.142 ± 0.0 g/100g respectively. The fat content had range of values from minimum to maximum 0.12 - 0.49 g/100g with mean±SD of 0.297 ± 0.11. Total sugar content varied between 66.2 - 71.8 g/100g with mean and S.D 68.88 ± 1.7. The sucrose content ranged between 0.60 - 4.28 g/100g with mean±SD 1.93 ± 0.8 while average value of fructose and glucose values were 36.84g/100g ± 0.9 and 30.41g/100g ± 3.00 respectively. All the tested samples showed very low content of Vitamin A while Vitamin C and flavonoids showed the average values of 112.45 ± 9.8 mg/kg and 43.0 ± 2.0 mg CE/kg respectively and polyphenols showed the average value of 748.76 ± 31.6 mg GAE/kg with 6057.5 - 887.2 mg GAE/kg min to max values. The % DPPH scavenging at 40mg/ml showed the values 61.8 - 75.7 min to max with the average of 70.3 ± 0.9. All the tested samples lie within the limits of international standard but Sample P4 showed the best results among all the 20 honey samples.

Page(s): 526-531

DOI: 10.36899/JAPS.2022.2.0450

Published: Journal: Journal of Animal and Plant sciences, Volume: 32, Issue: 2, Year: 2022

Keywords:

Flavonoids , antioxidant , antioxidant , physiochemical , Biochemical , composition , Honey , Total sugars

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