Abstract:
The major causes of death, acute myocardial infarction and ischemic stroke, are brought on by blood clots blocking blood vessels. The blood lump is liquefied by the administration of thrombolytic medications. This research involved the production of the thrombolytic agent fibrinolytic enzyme. Test sample were collected from laboratory of Enzyme Biotechnology Laboratory, Department of Biochemistry, University of Agriculture Faisalabad. Inoculated samples were placed onto nutrient agar medium plates, and Bacillus subtilis was used to extract the fibrinolytic enzyme. The substrates for the isolated bacterium were made of sugarcane bagasse. Bacterial strain was used for the production, propagation and purification of fibrinolytic enzyme. The enzyme was purified by ion exchange column chromatography. The protein was characterized using SDS-PAGE. To make fibrinolytic enzyme, an isolated strain of Bacillus subtilis was employed. A 307 U/ml enzyme activity with 62.65 U/mg specific activity, 4.9 mg/ml protein, 3.23 fold purification, and 82.52% recovery was generated by the isolated strain. The isolated strain's fibrinolytic enzyme was active between the pH ranges of 7.0 and 8.5; however it performed best at pH 7.0. The fibrinolytic enzyme demonstrated thermal stability between 37 and 39 degrees Celsius. It was concluded that Bacillus subtilis would develop to its maximum potential under these ideal conditions, enabling the industrial production of high-quality fibrinolytic enzyme at a low cost.
Page(s):
70-70
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Bacillus subtilis
,
Thrombolytic agent
,
Sugarcane bagasse
,
fibrinolytic enzyme