Whole Yeast Expressing Recombinant Fiber 2 Protein Vaccine Candidate Protects Chicken against Fowl Adenovirus Serotype 4 Infection | [Pakistan Veterinary Journal • 2020]

Author(s):

1. Van Dam Lai: Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University,Cheongju,South Korea

2. Joo-Sung Son: Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University,Cheongju,South Korea

3. Tea-Sik Kim: Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University,Cheongju,South Korea

4. Kang-San Kim: Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University,Cheongju,South Korea

5. Eui-Sung Choi: Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB),Cheongju,South Korea

6. In-Pil Mo: Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University,Cheongju, 361-763,South Korea

Abstract:

Fowl adenoviruses (FAdVs) are prevalent infectious agents in a variety of avian species including chickens worldwide. Among several serotypes, fowl adenovirus serotype 4 (FAdV-4) is known to cause mass economic damages to chicken farms in Korea as a pathogen for the hepatitis-hydropericardium syndrome (HHS). The encoding portions of hexon and fiber genes of FAdV-4 field strain ADL15 0401 were fully sequenced and three constructs, Hexon-L1-His and two constructs of fiber genes Fiber-1-His and Fiber-2-His were ligated with the pYEGa-HIR525a which was optimized for producing complex proteins in Saccharomyces cerevisiae yeast systems. The yeast cell containing these recombinant proteins were inoculated in specific pathogen-free (SPF) chickens. Antibodies against FAdVs were identified from all immunized chicken groups by commercial ELISA as early as 1 week post inoculation and better virus neutralization in Fiber-2 group with 2 logs neutralizing ability. Finally, the protective efficacy result showed the full protection after challenge with virulent FadV-4 virus in the SPF chicken from Fiber-1 and Fiber-2 groups. These results suggest that the whole yeast expressing recombinant fiber-2 protein can be a candidate for vaccines against the FAdV-4 infection.

Page(s): 49-54

DOI: 10.29261/pakvetj/2019.107

Published: Journal: Pakistan Veterinary Journal, Volume: 40, Issue: 1, Year: 2020

Keywords:

Fiber2 Hepatitishydropericardium syndrome Saccharomyces cerevisiae

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views